publication . Other literature type . 1996

Organization of photosystem I polypeptides examined by chemical cross-linking.

Armbrust, T S; Chitnis, P R; Guikema, J A;
Open Access English
  • Published: 01 Aug 1996
Abstract
Photosystem I from the cyanobacterium Synechocystis sp. PCC 6803 was examined using the chemical cross-linkers glutaraldehyde and N-ethyl-1-3-[3-(dimethylamino)propyl]carbodiimide to investigate the organization of the polypeptide subunits. Thylakoid membranes and photosystem I, which was isolated by Triton X-100 fractionation, were treated with cross-linking reagents and were resolved using a Tricine/urea low-molecular-weight resolution gel system. Subunit-specific antibodies and western blotting analysis were used to identify the components of cross-linked species. These analyses identified glutaraldehyde-dependent cross-linking products composed of small amou...
Subjects
Medical Subject Headings: polycyclic compounds
free text keywords: Research Article
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Other literature type . 1996
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