publication . Other literature type . Article . 2015

An Engineered Metal Sensor Tunes the Kinetics of Synaptic Transmission.

Evans, Chantell S.; Ruhl, David A.; Chapman, Edwin R.;
  • Published: 26 Aug 2015
  • Publisher: Society for Neuroscience
Abstract
The Ca 2+ sensor synaptotagmin-1 (syt-1) regulates neurotransmitter release by interacting with anionic phospholipids. Here we test the idea that the intrinsic kinetics of syt–membrane interactions determine, in part, the time course of synaptic transmission. To tune the kinetics of this interaction, we grafted structural elements from the slowest isoform, syt-7, onto the fastest isoform, syt-1, resulting in a chimera with intermediate kinetic properties. Moreover, the chimera coupled a physiologically irrelevant metal, Sr 2+ , to membrane fusion in vitro . When substituted for syt-1 in mouse hippocampal neurons, the chimera slowed the kinetics of synaptic trans...
Subjects
free text keywords: Neurotransmission, Biology, Synaptotagmin 1, Gene isoform, HEK 293 cells, Chimera (genetics), Exocytosis, Anatomy, Neurotransmitter, chemistry.chemical_compound, chemistry, Lipid bilayer fusion, Articles
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publication . Other literature type . Article . 2015

An Engineered Metal Sensor Tunes the Kinetics of Synaptic Transmission.

Evans, Chantell S.; Ruhl, David A.; Chapman, Edwin R.;