publication . Article . Other literature type . 2017

Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells

Requena, Sebastian; Ponomarchuk, Olga; Castillo, Marlius; Rebik, Jonathan; Brochiero, Emmanuelle; Borejdo, Julian; Gryczynski, Ignacy; Dzyuba, Sergei V.; Gryczynski, Zygmunt; Grygorczyk, Ryszard; ...
Open Access English
  • Published: 01 Dec 2017 Journal: Scientific Reports (issn: 2045-2322, Copyright policy)
  • Publisher: Nature Publishing Group
Abstract
Abstract Abnormalities of mucus viscosity play a critical role in the pathogenesis of several respiratory diseases, including cystic fibrosis. Currently, there are no approaches to assess the rheological properties of mucin granule matrices in live cells. This is the first example of the use of a molecular rotor, a BODIPY dye, to quantitatively visualize the viscosity of intragranular mucin matrices in a large population of individual granules in differentiated primary bronchial epithelial cells using fluorescence lifetime imaging microscopy.
Subjects
free text keywords: Medicine, R, Science, Q, Article, Population, education.field_of_study, education, BODIPY, chemistry.chemical_compound, chemistry, Mucin, Cystic fibrosis, medicine.disease, Fluorescence-lifetime imaging microscopy, Mucin granule, Granule (cell biology), Mucus, Biophysics
Funded by
NSF| Engineering Resonance Energy Transfer for Advanced Immunoassays
Project
  • Funder: National Science Foundation (NSF)
  • Project Code: 1264608
  • Funding stream: Directorate for Engineering | Division of Chemical, Bioengineering, Environmental, and Transport Systems
37 references, page 1 of 3

Elborn, JS. Cystic fibrosis. Lancet. 2016; 388: 2519-2531 [OpenAIRE] [PubMed] [DOI]

Ehre, C, Ridley, C, Thornton, DJ. Cystic fibrosis: an inherited disease affecting mucin-producing organs. Int. J. Biochem. Cell Biol.. 2014; 52: 136-145 [OpenAIRE] [PubMed] [DOI]

Boucher, RC. Cystic fibrosis: a disease of vulnerability to airway surface dehydration. Trends Mol. Med.. 2007; 13: 231-240 [OpenAIRE] [PubMed] [DOI]

Tang, XX. Acidic pH increases airway surface liquid viscosity in cystic fibrosis. J. Clin. Invest.. 2016; 126: 879-891 [OpenAIRE] [PubMed] [DOI]

Lai, SK, Wang, Y-Y, Wirtz, D, Hanes, J. Micro- and macrorheology of mucus. Adv. Drug Delivery Rev.. 2009; 61: 86-100 [OpenAIRE] [DOI]

Bansil, R, Stanley, E. & LaMont, Mucin biophysics. Annu. Rev. Physiol.. 1995; 57: 635-657 [OpenAIRE] [PubMed] [DOI]

7.Mucosal Immunology, 4th Ed., Eds: Mestecky, J., Strober, W., Russell, M.W., Cheroutre, H., Lambrecht, B.N. & Kelsall, B.L., 2015. Elsevier.

Bansil, R, Turner, BS. Mucin structure, aggregation, physiological functions and biomedical applications. Curr. Opin. Colloid Interface Sci.. 2006; 11: 164-170 [OpenAIRE] [DOI]

Klymchenko, AS. Solvatochromic and fluorogenic dyes as environment-sensitive probes: design and biological applications. Acc. Chem. Res.. 2017; 50: 366-375 [OpenAIRE] [PubMed] [DOI]

Kuimova, MK. Mapping viscosity in cells using molecular rotors. Phys. Chem. Chem. Phys.. 2012; 14: 12671-12686 [OpenAIRE] [PubMed] [DOI]

Sarder, P, Maji, D, Achilefu, S. Molecular Probes for Fluorescence Lifetime Imaging. Bioconjugate Chem.. 2015; 26: 963-974 [OpenAIRE] [DOI]

Becker, W. Fluorescence lifetime imaging – techniques and applications. J. Microscopy. 2012; 247: 119-136 [OpenAIRE] [DOI]

Berezin, MY, Achilefu, S. Fluorescence lifetime measurements and biological imaging. Chem. Rev.. 2010; 110: 2641-2684 [OpenAIRE] [PubMed] [DOI]

Kowada, T, Maeda, H, Kikuchi, K. BODIPY-based probes for the fluorescence imaging of biomolecules in living cells. Chem. Soc. Rev.. 2015; 44: 4953-4972 [OpenAIRE] [PubMed] [DOI]

Kamkaew, A. BODIPY dyes in photodynamic therapy. Chem. Soc. Rev.. 2013; 42: 77-88 [OpenAIRE] [PubMed] [DOI]

37 references, page 1 of 3
Abstract
Abstract Abnormalities of mucus viscosity play a critical role in the pathogenesis of several respiratory diseases, including cystic fibrosis. Currently, there are no approaches to assess the rheological properties of mucin granule matrices in live cells. This is the first example of the use of a molecular rotor, a BODIPY dye, to quantitatively visualize the viscosity of intragranular mucin matrices in a large population of individual granules in differentiated primary bronchial epithelial cells using fluorescence lifetime imaging microscopy.
Subjects
free text keywords: Medicine, R, Science, Q, Article, Population, education.field_of_study, education, BODIPY, chemistry.chemical_compound, chemistry, Mucin, Cystic fibrosis, medicine.disease, Fluorescence-lifetime imaging microscopy, Mucin granule, Granule (cell biology), Mucus, Biophysics
Funded by
NSF| Engineering Resonance Energy Transfer for Advanced Immunoassays
Project
  • Funder: National Science Foundation (NSF)
  • Project Code: 1264608
  • Funding stream: Directorate for Engineering | Division of Chemical, Bioengineering, Environmental, and Transport Systems
37 references, page 1 of 3

Elborn, JS. Cystic fibrosis. Lancet. 2016; 388: 2519-2531 [OpenAIRE] [PubMed] [DOI]

Ehre, C, Ridley, C, Thornton, DJ. Cystic fibrosis: an inherited disease affecting mucin-producing organs. Int. J. Biochem. Cell Biol.. 2014; 52: 136-145 [OpenAIRE] [PubMed] [DOI]

Boucher, RC. Cystic fibrosis: a disease of vulnerability to airway surface dehydration. Trends Mol. Med.. 2007; 13: 231-240 [OpenAIRE] [PubMed] [DOI]

Tang, XX. Acidic pH increases airway surface liquid viscosity in cystic fibrosis. J. Clin. Invest.. 2016; 126: 879-891 [OpenAIRE] [PubMed] [DOI]

Lai, SK, Wang, Y-Y, Wirtz, D, Hanes, J. Micro- and macrorheology of mucus. Adv. Drug Delivery Rev.. 2009; 61: 86-100 [OpenAIRE] [DOI]

Bansil, R, Stanley, E. & LaMont, Mucin biophysics. Annu. Rev. Physiol.. 1995; 57: 635-657 [OpenAIRE] [PubMed] [DOI]

7.Mucosal Immunology, 4th Ed., Eds: Mestecky, J., Strober, W., Russell, M.W., Cheroutre, H., Lambrecht, B.N. & Kelsall, B.L., 2015. Elsevier.

Bansil, R, Turner, BS. Mucin structure, aggregation, physiological functions and biomedical applications. Curr. Opin. Colloid Interface Sci.. 2006; 11: 164-170 [OpenAIRE] [DOI]

Klymchenko, AS. Solvatochromic and fluorogenic dyes as environment-sensitive probes: design and biological applications. Acc. Chem. Res.. 2017; 50: 366-375 [OpenAIRE] [PubMed] [DOI]

Kuimova, MK. Mapping viscosity in cells using molecular rotors. Phys. Chem. Chem. Phys.. 2012; 14: 12671-12686 [OpenAIRE] [PubMed] [DOI]

Sarder, P, Maji, D, Achilefu, S. Molecular Probes for Fluorescence Lifetime Imaging. Bioconjugate Chem.. 2015; 26: 963-974 [OpenAIRE] [DOI]

Becker, W. Fluorescence lifetime imaging – techniques and applications. J. Microscopy. 2012; 247: 119-136 [OpenAIRE] [DOI]

Berezin, MY, Achilefu, S. Fluorescence lifetime measurements and biological imaging. Chem. Rev.. 2010; 110: 2641-2684 [OpenAIRE] [PubMed] [DOI]

Kowada, T, Maeda, H, Kikuchi, K. BODIPY-based probes for the fluorescence imaging of biomolecules in living cells. Chem. Soc. Rev.. 2015; 44: 4953-4972 [OpenAIRE] [PubMed] [DOI]

Kamkaew, A. BODIPY dyes in photodynamic therapy. Chem. Soc. Rev.. 2013; 42: 77-88 [OpenAIRE] [PubMed] [DOI]

37 references, page 1 of 3
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