publication . Article . Doctoral thesis . 2001

Expression of MAGE-1 and -3 genes and gene products in human hepatocellular carcinoma

K Kariyama; T Higashi; Y Kobayashi; K Nouso; H Nakatsukasa; T Yamano; M Ishizaki; T Kaneyoshi; N Toshikuni; T Ohnishi; ...
Open Access English
  • Published: 25 Mar 2001 Journal: British Journal of Cancer, volume 81, issue 6, pages 1,080-1,087 (issn: 0007-0920, eissn: 1532-1827, Copyright policy)
  • Publisher: Nature Publishing Group
MAGE gene family encodes peptides recognized by autologous cytotoxic T lymphocytes in a major histocompatibility complex (MHC) class-I restricted fashion. In the present study, we have performed reverse-transcription polymerase chain reaction (RT-PCR) for the genes, as well as immunohistochemical analysis and Western blotting of MAGE-1 and -3 proteins in 33 surgically resected hepatocellular carcinomas (HCCs). MAGE-1 and -3 mRNAs were constitutively expressed exclusively in 78 and 42% of HCCs respectively. On immunohistochemistry with monoclonal antibodies, 77B for MAGE-1 and 57B for MAGE-3, MAGE-1 and -3 proteins were recognized in cytoplasm of only six among 3...
Medical Subject Headings: neoplasmsendocrine systemdigestive system diseases
free text keywords: Regular Article, tumour-rejection antigen, cancer testis antigen, immunohistochemistry, Western blotting, immunotherapy, Cancer Research, Oncology, Gene product, Gene expression, Major histocompatibility complex, biology.protein, biology, Pathology, medicine.medical_specialty, medicine, Western blot, medicine.diagnostic_test, HCCS, Hepatocellular carcinoma, medicine.disease, Blot, Immunology
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