publication . Article . Other literature type . 2012

microvesicles derived from human umbilical cord mesenchymal stem cells stimulated by hypoxia promote angiogenesis both in vitro and in vivo

Hong-Chao Zhang; Xin-Bin Liu; Shu Huang; Xiao-Yun Bi; Heng-Xiang Wang; Li-Xian Xie; Yong-Qi Wang; Xiao-Fang Cao; Jun Lv; Feng-Jun Xiao; ...
Open Access
  • Published: 10 Dec 2012 Journal: Stem Cells and Development, volume 21, pages 3,289-3,297 (issn: 1547-3287, eissn: 1557-8534, Copyright policy)
  • Publisher: Mary Ann Liebert Inc
Abstract
Although mesenchymal stem cells (MSCs) have been increasingly trialed to treat a variety of diseases, the underlying mechanisms remain still elusive. In this study, human umbilical cord (UC)-derived MSCs were stimulated by hypoxia, and the membrane microvesicles (MVs) in the supernatants were collected by ultracentrifugation, observed under an electron microscope, and the origin was identified with the flow cytometric technique. The results showed that upon hypoxic stimulus, MSCs released a large quantity of MVs of ∼100 nm in diameter. The MVs were phenotypically similar to the parent MSCs, except that the majority of them were negative for the receptor of plate...
Subjects
free text keywords: Cell biology, In vitro, Cellular differentiation, Angiogenesis, Mesenchymal stem cell, Growth factor, medicine.medical_treatment, medicine, Umbilical cord, medicine.anatomical_structure, Microvesicles, Flow cytometry, medicine.diagnostic_test, Biology, Original Research Reports
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publication . Article . Other literature type . 2012

microvesicles derived from human umbilical cord mesenchymal stem cells stimulated by hypoxia promote angiogenesis both in vitro and in vivo

Hong-Chao Zhang; Xin-Bin Liu; Shu Huang; Xiao-Yun Bi; Heng-Xiang Wang; Li-Xian Xie; Yong-Qi Wang; Xiao-Fang Cao; Jun Lv; Feng-Jun Xiao; ...