5-aza-2′-deoxycytidine-mediated c-myc Down-regulation Triggers Telomere-dependent Senescence by Regulating Human Telomerase Reverse Transcriptase in Chronic Myeloid Leukemia1

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Grandjenette, Cindy; Schnekenburger, Michael; Karius, Tommy; Ghelfi, Jenny; Gaigneaux, Anthoula; Henry, Estelle; Dicato, Mario; Diederich, Marc;
  • Publisher: Neoplasia Press
  • Journal: Neoplasia (New York, N.Y.),volume 16,issue 6,pages511-528 (issn: 1522-8002, eissn: 1476-5586)
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  • Related identifiers: doi: 10.1016/j.neo.2014.05.009, pmc: PMC4198755
  • Subject: hTERT, human telomerase reverse transcriptase | X-gal, 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside | CTCF, CCCTC-binding factor | DSB, double strand breaks | ChIP, chromatin immunoprecipitation | PBMC, peripheral blood mononuclear cell | Article | CML, chronic myeloid leukemia | SIPS, stress-induced premature senescence | FCS, fetal calf serum | TRAP, telomeric repeat amplification protocol | C12FDG, fluorogenic substrate 5-dodecanoylaminofluorescein di-β-D-galactopyranoside | DAC, 5-aza-2’-deoxycytidine | FITC, fluorescein isothiocyanate | SA-β-gal, senescence associated-β galactosidase | siRNA, small interfering RNA | CDKN, cyclin-dependent kinase inhibitor | M-PER®, Mammalian Protein Extraction Reagent | MSP, methylation-specific PCR

Increased proliferation rates as well as resistance to apoptosis are considered major obstacles for the treatment of patients with chronic myelogenous leukemia (CML), thus highlighting the need for novel therapeutic approaches. Since senescence has been recognized as a ... View more
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