publication . Article . 2014

Cyanidin 3-O-Glucoside Reduces Helicobacter pylori VacA-Induced Cell Death of Gastric KATO III Cells through Inhibition of the SecA Pathway

Kim, Sa-Hyun; Woo, Hyunjun; Park, Min; Rhee, Ki-Jong; Moon, Cheol; Lee, Dongsup; Seo, Woo Duck; Kim, Jong Bae;
Open Access
  • Published: 01 May 2014 Journal: International Journal of Medical Sciences, volume 11, pages 742-747 (issn: 1449-1907, Copyright policy)
  • Publisher: Ivyspring International Publisher
Abstract
Two key virulence factors of Helicobacter pylori are the secreted virulent proteins of vacuolating toxin A (VacA) and cytotoxin associated protein A (CagA) which lead to damages of gastric epithelial cells. We previously identified that the cyanidin 3-O-glucoside (C3G) inhibits the secretion of both VacA and CagA. In the current report, we show that C3G inhibits VacA secretion in a dose-dependent manner by inhibiting secretion system subunit protein A (SecA) synthesis. As SecA is involved in translocation of bacterial proteins, we predicted that inhibition of the SecA pathway by C3G should decrease H. pylori-induced cell death. To test this hypothesis, the human...
Subjects
Medical Subject Headings: bacterial infections and mycosesbacteriadigestive system diseases
free text keywords: General Medicine, Gastric mucosa, medicine.anatomical_structure, medicine, Molecular biology, Microbiology, Biology, Helicobacter pylori, biology.organism_classification, Secretion, Cell culture, Virulence, Cell damage, medicine.disease, Programmed cell death, CagA, Short Research Communication, H. pylori, cyanidin 3-O-glucoside, VacA secretion
Related Organizations
26 references, page 1 of 2

Kim, IJ, Blanke, SR. Remodeling the host environment: modulation of the gastric epithelium by the Helicobacter pylori vacuolating toxin (VacA). Front Cell Infect Microbiol . 2012; 2: 37 [OpenAIRE] [PubMed]

Oldani, A, Cormont, M, Hofman, V, Chiozzi, V, Oregioni, O, Canonici, A, Sciullo, A, Sommi, P, Fabbri, A, Ricci, V, Boquet, P. Helicobacter pylori counteracts the apoptotic action of its VacA toxin by injecting the CagA protein into gastric epithelial cells. PLoS Pathog . 2009; 5: e1000603 [OpenAIRE] [PubMed]

Cover, TL, Blanke, SR. Helicobacter pylori VacA, a paradigm for toxin Multifunctionality. Nat Rev Microbiol . 2005; 3: 320-332 [PubMed]

Rassow, J. Helicobacter pylori vacuolating toxin A and apoptosis. Cell Commun Signal . 2011; 9: 26 [OpenAIRE] [PubMed]

Wang, F, Xia, P, Wu, F, Wang, D, Wang, W, Ward, T, Liu, Y, Aikhionbare, F, Guo, Z, Powell, M, Liu, B, Bi, F, Shaw, A, Zhu, Z, Elmoselhi, A, Fan, D, Cover, TL, Ding, X, Yao, X. Helicobacter pylori VacA disrupts apical membrane-cytoskeletal interactions in gastric parietal cells. J Biol Chem . 2008; 283: 26714-26725 [OpenAIRE] [PubMed]

Chattopadhyay, R, Bhattacharyya, A, Crowe, SE. Dual Regulation by Apurinic/Apyrimidinic Endonuclease-1 Inhibits Gastric Epithelial Cell Apoptosis during Helicobacter pylori Infection. Cancer Res . 2010; 70: 2799-2808 [OpenAIRE] [PubMed]

Kumar Pachathundikandi, S, Brandt, S, Madassery, J, Backert, S. Induction of TLR-2 and TLR-5 expression by Helicobacter pylori switches cagPAI-dependent signalling leading to the secretion of IL-8 and TNF-α. PLoS ONE . 2011; 6: e19614 [OpenAIRE] [PubMed]

Schneider, S, Carra, G, Sahin, U, Hoy, B, Rieder, G, Wessler, S. Complex cellular responses of Helicobacter pylori-colonized gastric adenocarcinoma cells. Infect Immun . 2011; 79: 2362-2371 [OpenAIRE] [PubMed]

Franco, AT, Johnston, E, Krishna, U, Yamaoka, Y, Israel, DA, Nagy, TA, Wroblewski, LE, Piazuelo, MB, Correa, P, Peek, RM Jr. Regulation of gastric carcinogenesis by Helicobacter pylori virulence factors. Cancer Res . 2008; 68: 379-387 [OpenAIRE] [PubMed]

Gao, L, Michel, A, Weck, MN, Arndt, V, Pawlita, M, Brenner, H. Helicobacter pylori infection and gastric cancer risk: evaluation of 15 H. pylori proteins determined by novel multiplex serology. Cancer Res . 2009; 69: 6164-6170 [PubMed]

Keates, S, Keates, AC, Nath, S, Peek, RM Jr, Kelly, CP. Transactivation of the epidermal growth factor receptor by cag+ Helicobacter pylori induces upregulation of the early growth response gene Egr-1 in gastric epithelial cells. Gut . 2005; 54: 1363-1369 [OpenAIRE] [PubMed]

An, B, Lee, G, Lim, K, Moon, BS, Kim, JB. Identification of the repeated number of C and D regions of tyrosine phosphorylation motifs in Helicobacter pylori cagA using multiplex PCR. Microbiol Immunol . 2008; 52: 479-483 [PubMed]

Mueller, D, Tegtmeyer, N, Brandt, S, Yamaoka, Y, De Poire, E, Sgouras, D, Wessler, S, Torres, J, Smolka, A, Backert, S. c-Src and c-Abl kinases control hierarchic phosphorylation and function of the CagA effector protein in Western and East Asian Helicobacter pylori strains. J Clin Invest . 2012; 122: 1553-1566 [OpenAIRE] [PubMed]

Yang, JJ, Cho, LY, Ko, KP, Shin, A, Ma, SH, Choi, BY, Han, DS, Song, KS, Kim, YS, Lee, JY, Han, BG, Chang, SH, Shin, HR, Kang, D, Yoo, KY, Park, SK. Genetic susceptibility on CagA-interacting molecules and gene-environment interaction with phytoestrogens: a putative risk factor for gastric cancer. PLoS ONE . 2012; 7: e31020 [OpenAIRE] [PubMed]

Boquet, P, Ricci, V. Intoxication strategy of Helicobacter pylori VacA toxin. Trends in Microbiology . 2012; 20: 165-174 [OpenAIRE] [PubMed]

26 references, page 1 of 2
Abstract
Two key virulence factors of Helicobacter pylori are the secreted virulent proteins of vacuolating toxin A (VacA) and cytotoxin associated protein A (CagA) which lead to damages of gastric epithelial cells. We previously identified that the cyanidin 3-O-glucoside (C3G) inhibits the secretion of both VacA and CagA. In the current report, we show that C3G inhibits VacA secretion in a dose-dependent manner by inhibiting secretion system subunit protein A (SecA) synthesis. As SecA is involved in translocation of bacterial proteins, we predicted that inhibition of the SecA pathway by C3G should decrease H. pylori-induced cell death. To test this hypothesis, the human...
Subjects
Medical Subject Headings: bacterial infections and mycosesbacteriadigestive system diseases
free text keywords: General Medicine, Gastric mucosa, medicine.anatomical_structure, medicine, Molecular biology, Microbiology, Biology, Helicobacter pylori, biology.organism_classification, Secretion, Cell culture, Virulence, Cell damage, medicine.disease, Programmed cell death, CagA, Short Research Communication, H. pylori, cyanidin 3-O-glucoside, VacA secretion
Related Organizations
26 references, page 1 of 2

Kim, IJ, Blanke, SR. Remodeling the host environment: modulation of the gastric epithelium by the Helicobacter pylori vacuolating toxin (VacA). Front Cell Infect Microbiol . 2012; 2: 37 [OpenAIRE] [PubMed]

Oldani, A, Cormont, M, Hofman, V, Chiozzi, V, Oregioni, O, Canonici, A, Sciullo, A, Sommi, P, Fabbri, A, Ricci, V, Boquet, P. Helicobacter pylori counteracts the apoptotic action of its VacA toxin by injecting the CagA protein into gastric epithelial cells. PLoS Pathog . 2009; 5: e1000603 [OpenAIRE] [PubMed]

Cover, TL, Blanke, SR. Helicobacter pylori VacA, a paradigm for toxin Multifunctionality. Nat Rev Microbiol . 2005; 3: 320-332 [PubMed]

Rassow, J. Helicobacter pylori vacuolating toxin A and apoptosis. Cell Commun Signal . 2011; 9: 26 [OpenAIRE] [PubMed]

Wang, F, Xia, P, Wu, F, Wang, D, Wang, W, Ward, T, Liu, Y, Aikhionbare, F, Guo, Z, Powell, M, Liu, B, Bi, F, Shaw, A, Zhu, Z, Elmoselhi, A, Fan, D, Cover, TL, Ding, X, Yao, X. Helicobacter pylori VacA disrupts apical membrane-cytoskeletal interactions in gastric parietal cells. J Biol Chem . 2008; 283: 26714-26725 [OpenAIRE] [PubMed]

Chattopadhyay, R, Bhattacharyya, A, Crowe, SE. Dual Regulation by Apurinic/Apyrimidinic Endonuclease-1 Inhibits Gastric Epithelial Cell Apoptosis during Helicobacter pylori Infection. Cancer Res . 2010; 70: 2799-2808 [OpenAIRE] [PubMed]

Kumar Pachathundikandi, S, Brandt, S, Madassery, J, Backert, S. Induction of TLR-2 and TLR-5 expression by Helicobacter pylori switches cagPAI-dependent signalling leading to the secretion of IL-8 and TNF-α. PLoS ONE . 2011; 6: e19614 [OpenAIRE] [PubMed]

Schneider, S, Carra, G, Sahin, U, Hoy, B, Rieder, G, Wessler, S. Complex cellular responses of Helicobacter pylori-colonized gastric adenocarcinoma cells. Infect Immun . 2011; 79: 2362-2371 [OpenAIRE] [PubMed]

Franco, AT, Johnston, E, Krishna, U, Yamaoka, Y, Israel, DA, Nagy, TA, Wroblewski, LE, Piazuelo, MB, Correa, P, Peek, RM Jr. Regulation of gastric carcinogenesis by Helicobacter pylori virulence factors. Cancer Res . 2008; 68: 379-387 [OpenAIRE] [PubMed]

Gao, L, Michel, A, Weck, MN, Arndt, V, Pawlita, M, Brenner, H. Helicobacter pylori infection and gastric cancer risk: evaluation of 15 H. pylori proteins determined by novel multiplex serology. Cancer Res . 2009; 69: 6164-6170 [PubMed]

Keates, S, Keates, AC, Nath, S, Peek, RM Jr, Kelly, CP. Transactivation of the epidermal growth factor receptor by cag+ Helicobacter pylori induces upregulation of the early growth response gene Egr-1 in gastric epithelial cells. Gut . 2005; 54: 1363-1369 [OpenAIRE] [PubMed]

An, B, Lee, G, Lim, K, Moon, BS, Kim, JB. Identification of the repeated number of C and D regions of tyrosine phosphorylation motifs in Helicobacter pylori cagA using multiplex PCR. Microbiol Immunol . 2008; 52: 479-483 [PubMed]

Mueller, D, Tegtmeyer, N, Brandt, S, Yamaoka, Y, De Poire, E, Sgouras, D, Wessler, S, Torres, J, Smolka, A, Backert, S. c-Src and c-Abl kinases control hierarchic phosphorylation and function of the CagA effector protein in Western and East Asian Helicobacter pylori strains. J Clin Invest . 2012; 122: 1553-1566 [OpenAIRE] [PubMed]

Yang, JJ, Cho, LY, Ko, KP, Shin, A, Ma, SH, Choi, BY, Han, DS, Song, KS, Kim, YS, Lee, JY, Han, BG, Chang, SH, Shin, HR, Kang, D, Yoo, KY, Park, SK. Genetic susceptibility on CagA-interacting molecules and gene-environment interaction with phytoestrogens: a putative risk factor for gastric cancer. PLoS ONE . 2012; 7: e31020 [OpenAIRE] [PubMed]

Boquet, P, Ricci, V. Intoxication strategy of Helicobacter pylori VacA toxin. Trends in Microbiology . 2012; 20: 165-174 [OpenAIRE] [PubMed]

26 references, page 1 of 2
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publication . Article . 2014

Cyanidin 3-O-Glucoside Reduces Helicobacter pylori VacA-Induced Cell Death of Gastric KATO III Cells through Inhibition of the SecA Pathway

Kim, Sa-Hyun; Woo, Hyunjun; Park, Min; Rhee, Ki-Jong; Moon, Cheol; Lee, Dongsup; Seo, Woo Duck; Kim, Jong Bae;