publication . Article . Other literature type . 2018

RNA Interference (RNAi) Screening in Drosophila

Heigwer, Florian; Port, Fillip; Boutros, Michael;
Open Access English
  • Published: 27 Feb 2018 Journal: Genetics, volume 208, issue 3, pages 853-874 (issn: 0016-6731, eissn: 1943-2631, Copyright policy)
  • Publisher: Genetics Society of America
Abstract
In the last decade, RNA interference (RNAi), a cellular mechanism that uses RNA-guided degradation of messenger RNA transcripts, has had an important impact on identifying and characterizing gene function. First discovered in Caenorhabditis elegans, RNAi can be used to silence the expression of genes through introduction of exogenous double-stranded RNA into cells. In Drosophila, RNAi has been applied in cultured cells or in vivo to perturb the function of single genes or to systematically probe gene function on a genome-wide scale. In this review, we will describe the use of RNAi to study gene function in Drosophila with a particular focus on high-throughput sc...
Subjects
free text keywords: FlyBook, Methods, Drosophila, RNAi, functional genomics, high-throughput screening, image-based screening, bioinformatics, genome engineering, Genetics, Gene, Gene knockdown, Cas9, Biology, Caenorhabditis elegans, biology.organism_classification, Computational biology, RNA interference, CRISPR, RNA
211 references, page 1 of 15

Alalouf G.Hess A.Costigan M.Keene A. C.Goulas S., 2010 A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.Cell 143: 628–638.21074052 [OpenAIRE] [PubMed]

Alic N.Hoddinott M. P.Foley A.Slack C.Piper M. D. W., 2012 Detrimental effects of RNAi: a cautionary note on its use in Drosophila ageing studies.PLoS One 7: e45367.23028964 [OpenAIRE] [PubMed]

Ameres S. L.Zamore P. D., 2013 Diversifying microRNA sequence and function.Nat. Rev. Mol. Cell Biol.14: 475–488.23800994 [OpenAIRE] [PubMed]

Arziman Z.Horn T.Boutros M., 2005 E-RNAi: a web application to design optimized RNAi constructs.Nucleic Acids Res.33: W582–W588.15980541 [OpenAIRE] [PubMed]

Ashton-Beaucage D.Udell C. M.Gendron P.Sahmi M.Lefrançois M., 2014 A functional screen reveals an extensive layer of transcriptional and splicing control underlying RAS/MAPK signaling in Drosophila.PLoS Biol.12: e1001809.24643257 [OpenAIRE] [PubMed]

Baeg G.-H.Zhou R.Perrimon N., 2005 Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila.Genes Dev.19: 1861–1870.16055650 [OpenAIRE] [PubMed]

Bai J.Sepp K. J.Perrimon N., 2009 Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screening.Nat. Protoc.4: 1502–1512.19798083 [OpenAIRE] [PubMed]

Bai S. W.Herrera-Abreu M. T.Rohn J. L.Racine V.Tajadura V., 2011 Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration.BMC B iol.9: 54.21834987 [OpenAIRE] [PubMed]

Bakal C.Linding R.Llense F.Heffern E.Martin-Blanco E., 2008 Phosphorylation networks regulating JNK activity in diverse genetic backgrounds.Science 322: 453–456.18927396 [OpenAIRE] [PubMed]

Bard F.Casano L.Mallabiabarrena A.Wallace E.Saito K., 2006 Functional genomics reveals genes involved in protein secretion and Golgi organization.Nature 439: 604–607.16452979 [OpenAIRE] [PubMed]

Barrangou R.Fremaux C.Deveau H.Richards M.Boyaval P., 2007 CRISPR provides acquired resistance against viruses in prokaryotes.Science 315: 1709–1712.17379808 [OpenAIRE] [PubMed]

Bartscherer K.Pelte N.Ingelfinger D.Boutros M., 2006 Secretion of Wnt ligands requires Evi, a conserved transmembrane protein.Cell 125: 523–533.16678096 [OpenAIRE] [PubMed]

Bassett A. R.Tibbit C.Ponting C. P.Liu J.-L., 2013 Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system.Cell Rep.4: 220–228.23827738 [OpenAIRE] [PubMed]

Baum B.Cherbas L., 2008 Drosophila cell lines as model systems and as an experimental tool.Methods Mol. Biol.420: 391–424.18641962 [OpenAIRE] [PubMed]

Bettencourt-Dias M.Giet R.Sinka R.Mazumdar A.Lock W. G., 2004 Genome-wide survey of protein kinases required for cell cycle progression.Nature 432: 980–987.15616552 [OpenAIRE] [PubMed]

211 references, page 1 of 15
Abstract
In the last decade, RNA interference (RNAi), a cellular mechanism that uses RNA-guided degradation of messenger RNA transcripts, has had an important impact on identifying and characterizing gene function. First discovered in Caenorhabditis elegans, RNAi can be used to silence the expression of genes through introduction of exogenous double-stranded RNA into cells. In Drosophila, RNAi has been applied in cultured cells or in vivo to perturb the function of single genes or to systematically probe gene function on a genome-wide scale. In this review, we will describe the use of RNAi to study gene function in Drosophila with a particular focus on high-throughput sc...
Subjects
free text keywords: FlyBook, Methods, Drosophila, RNAi, functional genomics, high-throughput screening, image-based screening, bioinformatics, genome engineering, Genetics, Gene, Gene knockdown, Cas9, Biology, Caenorhabditis elegans, biology.organism_classification, Computational biology, RNA interference, CRISPR, RNA
211 references, page 1 of 15

Alalouf G.Hess A.Costigan M.Keene A. C.Goulas S., 2010 A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.Cell 143: 628–638.21074052 [OpenAIRE] [PubMed]

Alic N.Hoddinott M. P.Foley A.Slack C.Piper M. D. W., 2012 Detrimental effects of RNAi: a cautionary note on its use in Drosophila ageing studies.PLoS One 7: e45367.23028964 [OpenAIRE] [PubMed]

Ameres S. L.Zamore P. D., 2013 Diversifying microRNA sequence and function.Nat. Rev. Mol. Cell Biol.14: 475–488.23800994 [OpenAIRE] [PubMed]

Arziman Z.Horn T.Boutros M., 2005 E-RNAi: a web application to design optimized RNAi constructs.Nucleic Acids Res.33: W582–W588.15980541 [OpenAIRE] [PubMed]

Ashton-Beaucage D.Udell C. M.Gendron P.Sahmi M.Lefrançois M., 2014 A functional screen reveals an extensive layer of transcriptional and splicing control underlying RAS/MAPK signaling in Drosophila.PLoS Biol.12: e1001809.24643257 [OpenAIRE] [PubMed]

Baeg G.-H.Zhou R.Perrimon N., 2005 Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila.Genes Dev.19: 1861–1870.16055650 [OpenAIRE] [PubMed]

Bai J.Sepp K. J.Perrimon N., 2009 Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screening.Nat. Protoc.4: 1502–1512.19798083 [OpenAIRE] [PubMed]

Bai S. W.Herrera-Abreu M. T.Rohn J. L.Racine V.Tajadura V., 2011 Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration.BMC B iol.9: 54.21834987 [OpenAIRE] [PubMed]

Bakal C.Linding R.Llense F.Heffern E.Martin-Blanco E., 2008 Phosphorylation networks regulating JNK activity in diverse genetic backgrounds.Science 322: 453–456.18927396 [OpenAIRE] [PubMed]

Bard F.Casano L.Mallabiabarrena A.Wallace E.Saito K., 2006 Functional genomics reveals genes involved in protein secretion and Golgi organization.Nature 439: 604–607.16452979 [OpenAIRE] [PubMed]

Barrangou R.Fremaux C.Deveau H.Richards M.Boyaval P., 2007 CRISPR provides acquired resistance against viruses in prokaryotes.Science 315: 1709–1712.17379808 [OpenAIRE] [PubMed]

Bartscherer K.Pelte N.Ingelfinger D.Boutros M., 2006 Secretion of Wnt ligands requires Evi, a conserved transmembrane protein.Cell 125: 523–533.16678096 [OpenAIRE] [PubMed]

Bassett A. R.Tibbit C.Ponting C. P.Liu J.-L., 2013 Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system.Cell Rep.4: 220–228.23827738 [OpenAIRE] [PubMed]

Baum B.Cherbas L., 2008 Drosophila cell lines as model systems and as an experimental tool.Methods Mol. Biol.420: 391–424.18641962 [OpenAIRE] [PubMed]

Bettencourt-Dias M.Giet R.Sinka R.Mazumdar A.Lock W. G., 2004 Genome-wide survey of protein kinases required for cell cycle progression.Nature 432: 980–987.15616552 [OpenAIRE] [PubMed]

211 references, page 1 of 15
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publication . Article . Other literature type . 2018

RNA Interference (RNAi) Screening in Drosophila

Heigwer, Florian; Port, Fillip; Boutros, Michael;