publication . Article . 2014

Optimized Scorpion Polypeptide LMX: A Pest Control Protein Effective against Rice Leaf Folder

Xiuzi Tianpei; Yingguo Zhu; Shaoqing Li;
Open Access English
  • Published: 25 Jun 2014 Journal: PLoS ONE (issn: 1932-6203, Copyright policy)
  • Publisher: Public Library of Science (PLoS)
Abstract
Lepidopteran insect pests are the main class of pests causing significant damage to crop plant yields. Insecticidal scorpion peptides exhibit toxicity specific for insects. Here, we report that a peptide LMX, optimized from the insect-specific scorpion neurotoxin LqhIT2, showed high levels of activity against rice leaf folder in vitro and in planta. Oral ingestion of LMX protein led to a significant decrease in feeding on rice leaves, repression of larval growth and development, delay in molting, and increase in larval lethality. Compared with LqhIT2 protein, the stability and insecticidal efficacy of LMX was better. Meanwhile, biochemical analysis showed that L...
Subjects
Medical Subject Headings: food and beveragesfungi
free text keywords: Medicine, R, Science, Q, Research Article, Biology and Life Sciences, Agriculture, Agrochemicals, Pesticides, Crops, Cereal Crops, Rice, Agricultural Biotechnology, Pest Control, Biotechnology, Bioengineering, Plant Science, Plant Pathology, Plant Pests, Toxicology, Toxic Agents, Engineering and Technology, General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, General Medicine, Chymotrypsin, biology.protein, biology, Transgene, Ecdysone, chemistry.chemical_compound, chemistry, Oryza sativa, Biochemistry, Genetically modified crops, Botany, Wild type, Catalase, Glutathione
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52 references, page 1 of 4

1 Food and Agriculture Organization of the United Nations (FAO) (2004) The state of food security in the world. pp. 30–31.

2 Khush GS, Brar DS (2002) Biotechnology for rice breeding: progress and potential impact. In: Proc. 20th Session of the Int. Rice Commission: 23 rd–26 th July 2002; Thailand: Bangkok.

3 Luo SJ (2010) Occurrence of rice leaf roller in China and its identification and prevention. Plant Diseases and Pests 1: 13–18.

4 Alvi SM, Ali MA, Chaudhary S, Iqbal S (2003) Population trends and chemical control of rice leaf folder, Cnaphalocrocis medinalis on rice crop. Int J Agri Biol 5: 615–617.

5 Li SW, Yang H, Liu YF, Liao QR, Du J, et al (2012) Transcriptome and gene expression analysis of the rice leaf folder, Cnaphalocrosis medinalis. PLoS One 7: e47401.23185238 [OpenAIRE] [PubMed]

6 Ho NH, Baisakh N, Oliva N, Datta K, Frutos R, et al (2006) Translation fusion Hybrid Bt genes confer resis tance against yellow stem borer in transgenic elite Vietnamese rice (Oryza sativa L.) cultivars. Crop Sci 46: 781–789. [OpenAIRE]

7 Deka S, Barthakur S (2010) Overview on current status of biotechnological interventions on yellow stem borer Scirpophaga incertulas (Lepidoptera: Crambidae) resistance in rice. Biotechnol Adv 28: 70–81.19811767 [PubMed]

8 Kumar S, Chandra A, Pandey KC (2008) Bacillus thuringiensis (Bt) transgenic crop: an environment friendly insect-pest management strategy. J Environ Biol 29: 641–653.19295059 [PubMed]

9 Shelton AM, Zhao JZ, Roush RT (2002) Economic, ecological, food, safety and social consequences of the deployment of Bt transgenic plants. Annu Rev Entomol 47: 845–881.11729093 [PubMed]

10 Froy O, Sagiv T, Poreh M, Urbach D, Zilberberg N, et al (1999) Dynamic diversification from a pupative common ancestor of scorpion toxins affecting sodium, potassium and chloride channels. J Mol Evol 48: 187–196.9929387 [OpenAIRE] [PubMed]

11 Gurevitz M, Karbat I, Cohen L, Ilan N, Kahn R, et al (2007) The insecticidal potential of scorpion β-toxins. Toxicon 49: 473–489.17197009 [PubMed]

12 Bergeron ZL, Bingham JP (2012) Scorpion toxins specific for potassium (K+) channels: a historical overview of peptide bioengineering. Toxins 4: 1082–1119.23202307 [OpenAIRE] [PubMed]

13 Rodríguez de la Vega RC, Possani LD (2005) Overview of scorpion toxins specific for Na+-channels and related peptides: biodiversity, structure-function relationships and evolution. Toxicon 46: 831–844.16274721 [OpenAIRE] [PubMed]

14 van Beek N, Lu A, Presnail J, Davis D, Greenamoyer C, et al (2003) Effect of signal sequence and promoter on the speed of action of a genetically modified Autographa californica nucleopolyhedrovirus expressing the scorpion toxin LqhIT2. Biol Control 27: 53–64.

15 Regev A, Rivkin H, Inceoglu B, Gershburg E, Hammock BD, et al (2003) Further enhancement of baculovirus insecticidal efficacy with scorpion toxins that interact cooperatively. FEBS Lett 537: 106–110.12606040 [OpenAIRE] [PubMed]

52 references, page 1 of 4
Abstract
Lepidopteran insect pests are the main class of pests causing significant damage to crop plant yields. Insecticidal scorpion peptides exhibit toxicity specific for insects. Here, we report that a peptide LMX, optimized from the insect-specific scorpion neurotoxin LqhIT2, showed high levels of activity against rice leaf folder in vitro and in planta. Oral ingestion of LMX protein led to a significant decrease in feeding on rice leaves, repression of larval growth and development, delay in molting, and increase in larval lethality. Compared with LqhIT2 protein, the stability and insecticidal efficacy of LMX was better. Meanwhile, biochemical analysis showed that L...
Subjects
Medical Subject Headings: food and beveragesfungi
free text keywords: Medicine, R, Science, Q, Research Article, Biology and Life Sciences, Agriculture, Agrochemicals, Pesticides, Crops, Cereal Crops, Rice, Agricultural Biotechnology, Pest Control, Biotechnology, Bioengineering, Plant Science, Plant Pathology, Plant Pests, Toxicology, Toxic Agents, Engineering and Technology, General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, General Medicine, Chymotrypsin, biology.protein, biology, Transgene, Ecdysone, chemistry.chemical_compound, chemistry, Oryza sativa, Biochemistry, Genetically modified crops, Botany, Wild type, Catalase, Glutathione
Related Organizations
Download fromView all 3 versions
PLoS ONE
Article . 2014
PLoS ONE
Article . 2014
Provider: Crossref
PLoS ONE
Article
Provider: UnpayWall
52 references, page 1 of 4

1 Food and Agriculture Organization of the United Nations (FAO) (2004) The state of food security in the world. pp. 30–31.

2 Khush GS, Brar DS (2002) Biotechnology for rice breeding: progress and potential impact. In: Proc. 20th Session of the Int. Rice Commission: 23 rd–26 th July 2002; Thailand: Bangkok.

3 Luo SJ (2010) Occurrence of rice leaf roller in China and its identification and prevention. Plant Diseases and Pests 1: 13–18.

4 Alvi SM, Ali MA, Chaudhary S, Iqbal S (2003) Population trends and chemical control of rice leaf folder, Cnaphalocrocis medinalis on rice crop. Int J Agri Biol 5: 615–617.

5 Li SW, Yang H, Liu YF, Liao QR, Du J, et al (2012) Transcriptome and gene expression analysis of the rice leaf folder, Cnaphalocrosis medinalis. PLoS One 7: e47401.23185238 [OpenAIRE] [PubMed]

6 Ho NH, Baisakh N, Oliva N, Datta K, Frutos R, et al (2006) Translation fusion Hybrid Bt genes confer resis tance against yellow stem borer in transgenic elite Vietnamese rice (Oryza sativa L.) cultivars. Crop Sci 46: 781–789. [OpenAIRE]

7 Deka S, Barthakur S (2010) Overview on current status of biotechnological interventions on yellow stem borer Scirpophaga incertulas (Lepidoptera: Crambidae) resistance in rice. Biotechnol Adv 28: 70–81.19811767 [PubMed]

8 Kumar S, Chandra A, Pandey KC (2008) Bacillus thuringiensis (Bt) transgenic crop: an environment friendly insect-pest management strategy. J Environ Biol 29: 641–653.19295059 [PubMed]

9 Shelton AM, Zhao JZ, Roush RT (2002) Economic, ecological, food, safety and social consequences of the deployment of Bt transgenic plants. Annu Rev Entomol 47: 845–881.11729093 [PubMed]

10 Froy O, Sagiv T, Poreh M, Urbach D, Zilberberg N, et al (1999) Dynamic diversification from a pupative common ancestor of scorpion toxins affecting sodium, potassium and chloride channels. J Mol Evol 48: 187–196.9929387 [OpenAIRE] [PubMed]

11 Gurevitz M, Karbat I, Cohen L, Ilan N, Kahn R, et al (2007) The insecticidal potential of scorpion β-toxins. Toxicon 49: 473–489.17197009 [PubMed]

12 Bergeron ZL, Bingham JP (2012) Scorpion toxins specific for potassium (K+) channels: a historical overview of peptide bioengineering. Toxins 4: 1082–1119.23202307 [OpenAIRE] [PubMed]

13 Rodríguez de la Vega RC, Possani LD (2005) Overview of scorpion toxins specific for Na+-channels and related peptides: biodiversity, structure-function relationships and evolution. Toxicon 46: 831–844.16274721 [OpenAIRE] [PubMed]

14 van Beek N, Lu A, Presnail J, Davis D, Greenamoyer C, et al (2003) Effect of signal sequence and promoter on the speed of action of a genetically modified Autographa californica nucleopolyhedrovirus expressing the scorpion toxin LqhIT2. Biol Control 27: 53–64.

15 Regev A, Rivkin H, Inceoglu B, Gershburg E, Hammock BD, et al (2003) Further enhancement of baculovirus insecticidal efficacy with scorpion toxins that interact cooperatively. FEBS Lett 537: 106–110.12606040 [OpenAIRE] [PubMed]

52 references, page 1 of 4
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publication . Article . 2014

Optimized Scorpion Polypeptide LMX: A Pest Control Protein Effective against Rice Leaf Folder

Xiuzi Tianpei; Yingguo Zhu; Shaoqing Li;