publication . Article . 2012

Transcriptome Analysis of Responses to Rhodomyrtone in Methicillin-Resistant Staphylococcus aureus

Wipawadee Sianglum; Potjanee Srimanote; Peter W Taylor; Helena Rosado; Supayang P Voravuthikunchai;
Open Access
  • Published: 27 Sep 2012 Journal: PLoS ONE, volume 7, page e45744 (eissn: 1932-6203, Copyright policy)
  • Publisher: Public Library of Science (PLoS)
  • Country: United Kingdom
Abstract
Rhodomyrtone, purified from Rhodomyrtus tomentosa (Aiton) Hassk, exhibits a high degree of potency against methicillin-resistant Staphylococcus aureus (MRSA). We recently demonstrated that exposure of MRSA to a subinhibitory concentration (0.174 µg/ml) of rhodomyrtone resulted in the alteration of expression of several functional classes of bacterial proteins. To provide further insight into the antibacterial mode of action of this compound, we determined the impact of exposure to rhodomyrtone on the gene transcriptional profile of MRSA using microarray analysis. Exposure of MRSA to subinhibitory concentrations (0.5MIC; 0.5 µg/ml) of rhodomyrtone revealed signif...
Subjects
free text keywords: General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, General Medicine, Amino acid, chemistry.chemical_classification, chemistry, Peptidoglycan, chemistry.chemical_compound, Biology, Biosynthesis, Microbiology, Gene expression, Mode of action, Operon, Metabolic pathway, Staphylococcus aureus, medicine.disease_cause, medicine, Biochemistry, Research Article, Computational Biology, Genomics, Genome Analysis Tools, Transcriptomes, Bacterial Pathogens, Staphylococci, Infectious diseases, Bacterial diseases, Methicillin-resistant Staphylococcus aureus, R, Science, Q
32 references, page 1 of 3

1. Chambers HF (1988) Methicillin-resistant staphylococci. Clin Microbiol Rev 1: 173-186.

2. Wenzel RP, Bearman G, Edmond MB (2007) Community-acquired methicillinresistant Staphylococcus aureus (MRSA): new issues for infection control. Int J Antimicrob Agents 30: 210-212. [OpenAIRE]

3. Lyon BR, Skurray R (1987) Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev 51: 88-134. [OpenAIRE]

4. Aksoy DY, Unal S (2008) New antimicrobial agents for the treatment of Grampositive bacterial infections. Clin Microbiol Infect 14: 411-420.

5. Eliopoulos GM (2009) Microbiology of drugs for treating multiply drug-resistant Gram-positive bacteria. J Infect 59 Suppl 1: S17-24. [OpenAIRE]

6. Shakil S, Akram M, Khan AU (2008) Tigecycline: a critical update. J Chemother 20: 411-419.

7. Sorlozano A, Gutierrez J, Martinez T, Yuste ME, Perez-Lopez JA, et al. (2010) Detection of new mutations conferring resistance to linezolid in glycopeptideintermediate susceptibility Staphylococcus hominis subspecies hominis circulating in an intensive care unit. Eur J Clin Microbiol Infect Dis 29: 73-80.

8. Bradley JS (2005) Newer antistaphylococcal agents. Curr Opin Pediatr 17: 71- 77.

9. Gibbons S (2004) Anti-staphylococcal plant natural products. Nat Prod Rep 21: 263-277.

10. Sass V, Pag U, Tossi A, Bierbaum G, Sahl HG (2008) Mode of action of human beta-defensin 3 against Staphylococcus aureus and transcriptional analysis of responses to defensin challenge. Int J Med Microbiol 298: 619-633. [OpenAIRE]

11. Martin KW, Ernst E (2003) Herbal medicines for treatment of bacterial infections: a review of controlled clinical trials. J Antimicrob Chemother 51: 241-246.

12. Cowan MM (1999) Plant products as antimicrobial agents. Clin Microbiol Rev 12: 564-582.

13. Samy RP, Gopalakrishnakone P (2008) Therapeutic Potential of Plants as Antimicrobials for Drug Discovery. Evid Based Complement Alternat Med: 283- 294. [OpenAIRE]

14. Dachriyanus Salni, Sargent MV, Skelton BW, Soediro I, et al. (2002) Rhodomyrtone, an antibiotic from Rhodomyrtus tomentosa. Aust J Chem 55: 229-232.

15. Hiranrat A, Mahabusarakam W (2008) New acylphloroglucinols from the leaves of Rhodomyrtus tomentosa. Tetrahedron 64: 11193-11197. [OpenAIRE]

32 references, page 1 of 3
Abstract
Rhodomyrtone, purified from Rhodomyrtus tomentosa (Aiton) Hassk, exhibits a high degree of potency against methicillin-resistant Staphylococcus aureus (MRSA). We recently demonstrated that exposure of MRSA to a subinhibitory concentration (0.174 µg/ml) of rhodomyrtone resulted in the alteration of expression of several functional classes of bacterial proteins. To provide further insight into the antibacterial mode of action of this compound, we determined the impact of exposure to rhodomyrtone on the gene transcriptional profile of MRSA using microarray analysis. Exposure of MRSA to subinhibitory concentrations (0.5MIC; 0.5 µg/ml) of rhodomyrtone revealed signif...
Subjects
free text keywords: General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, General Medicine, Amino acid, chemistry.chemical_classification, chemistry, Peptidoglycan, chemistry.chemical_compound, Biology, Biosynthesis, Microbiology, Gene expression, Mode of action, Operon, Metabolic pathway, Staphylococcus aureus, medicine.disease_cause, medicine, Biochemistry, Research Article, Computational Biology, Genomics, Genome Analysis Tools, Transcriptomes, Bacterial Pathogens, Staphylococci, Infectious diseases, Bacterial diseases, Methicillin-resistant Staphylococcus aureus, R, Science, Q
32 references, page 1 of 3

1. Chambers HF (1988) Methicillin-resistant staphylococci. Clin Microbiol Rev 1: 173-186.

2. Wenzel RP, Bearman G, Edmond MB (2007) Community-acquired methicillinresistant Staphylococcus aureus (MRSA): new issues for infection control. Int J Antimicrob Agents 30: 210-212. [OpenAIRE]

3. Lyon BR, Skurray R (1987) Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev 51: 88-134. [OpenAIRE]

4. Aksoy DY, Unal S (2008) New antimicrobial agents for the treatment of Grampositive bacterial infections. Clin Microbiol Infect 14: 411-420.

5. Eliopoulos GM (2009) Microbiology of drugs for treating multiply drug-resistant Gram-positive bacteria. J Infect 59 Suppl 1: S17-24. [OpenAIRE]

6. Shakil S, Akram M, Khan AU (2008) Tigecycline: a critical update. J Chemother 20: 411-419.

7. Sorlozano A, Gutierrez J, Martinez T, Yuste ME, Perez-Lopez JA, et al. (2010) Detection of new mutations conferring resistance to linezolid in glycopeptideintermediate susceptibility Staphylococcus hominis subspecies hominis circulating in an intensive care unit. Eur J Clin Microbiol Infect Dis 29: 73-80.

8. Bradley JS (2005) Newer antistaphylococcal agents. Curr Opin Pediatr 17: 71- 77.

9. Gibbons S (2004) Anti-staphylococcal plant natural products. Nat Prod Rep 21: 263-277.

10. Sass V, Pag U, Tossi A, Bierbaum G, Sahl HG (2008) Mode of action of human beta-defensin 3 against Staphylococcus aureus and transcriptional analysis of responses to defensin challenge. Int J Med Microbiol 298: 619-633. [OpenAIRE]

11. Martin KW, Ernst E (2003) Herbal medicines for treatment of bacterial infections: a review of controlled clinical trials. J Antimicrob Chemother 51: 241-246.

12. Cowan MM (1999) Plant products as antimicrobial agents. Clin Microbiol Rev 12: 564-582.

13. Samy RP, Gopalakrishnakone P (2008) Therapeutic Potential of Plants as Antimicrobials for Drug Discovery. Evid Based Complement Alternat Med: 283- 294. [OpenAIRE]

14. Dachriyanus Salni, Sargent MV, Skelton BW, Soediro I, et al. (2002) Rhodomyrtone, an antibiotic from Rhodomyrtus tomentosa. Aust J Chem 55: 229-232.

15. Hiranrat A, Mahabusarakam W (2008) New acylphloroglucinols from the leaves of Rhodomyrtus tomentosa. Tetrahedron 64: 11193-11197. [OpenAIRE]

32 references, page 1 of 3
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publication . Article . 2012

Transcriptome Analysis of Responses to Rhodomyrtone in Methicillin-Resistant Staphylococcus aureus

Wipawadee Sianglum; Potjanee Srimanote; Peter W Taylor; Helena Rosado; Supayang P Voravuthikunchai;