publication . Other literature type . Article . 1996

The NAD(P)H-utilizing glutamate dehydrogenase of Bacteroides thetaiotaomicron belongs to enzyme family I, and its activity is affected by trans-acting gene(s) positioned downstream of gdhA.

L Baggio; M Morrison;
Open Access English
  • Published: 01 Dec 1996
Previous studies have suggested that regulation of the enzymes of ammonia assimilation in human colonic Bacteroides species is coordinated differently than in other eubacteria. The gene encoding an NAD(P)H-dependent glutamate dehydrogenase (gdhA) in Bacteroides thetaiotaomicron was cloned and expressed in Escherichia coli by mutant complementation from the recombinant plasmid pANS100. Examination of the predicted GdhA amino acid sequence revealed that this enzyme possesses motifs typical of the family I-type hexameric GDH proteins. Northern blot analysis with a gdhA-specific probe indicated that a single transcript with an electrophoretic mobility of approximate...
free text keywords: Research Article, Molecular Biology, Microbiology, Gene product, Plasmid, Mutant, Gene, Insertional mutagenesis, Complementation, Glutamate dehydrogenase, Biochemistry, Escherichia coli, medicine.disease_cause, medicine, Biology
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