publication . Article . 2015

Responsiveness of platelets during storage studied with flow cytometry--formation of platelet subpopulations and LAMP-1 as new markers for the platelet storage lesion.

Gösta Berlin; Anna L. Södergren; Sofia Ramström; Nahreen Tynngård;
Open Access
  • Published: 21 Sep 2015 Journal: Vox Sanguinis, volume 110, pages 116-125 (issn: 0042-9007, Copyright policy)
  • Publisher: Wiley
  • Country: Sweden
Abstract
Background and ObjectivesStorage lesions may prevent transfused platelets to respond to agonists and arrest bleeding. The aim of this study was to evaluate and quantify the capacity of platelet activation during storage using flow cytometry and new markers of platelet activation. Materials and MethodsActivation responses of platelets prepared by apheresis were measured on days 1, 5, 7 and 12. In addition, comparisons were made for platelet concentrates stored until swirling was affected. Lysosome-associated membrane protein-1 (LAMP-1), P-selectin and phosphatidylserine (PS) exposure were assessed by flow cytometry on platelets in different subpopulations in rest...
Subjects
free text keywords: Hematology, General Medicine, apheresis; haemostasis; platelet concentrates; platelet function; platelet transfusion, Clinical Medicine, Klinisk medicin, Immunology, Biomarker (medicine), Lysosomal-Associated Membrane Protein 1, Pathology, medicine.medical_specialty, medicine, Flow cytometry, medicine.diagnostic_test, Lesion, medicine.symptom, Platelet storage, Platelet activation, business.industry, business, Platelet, Platelet transfusion
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