publication . Doctoral thesis . 2008

Analysis of Rca1 function at the G1-S transition in Drosophila melanogaster

Querings, Silvia;
Open Access English
  • Published: 01 Jan 2008
  • Country: Germany
Abstract
Tight control of APC/C-Cdh1Fzr activity is essential for progression through mitosis and establishment of the G1 phase. Rca1 is a nuclear protein that inhibits the APC/C-Cdh1Fzr complex during G2 to allow cyclin accumulation and subsequent entry into mitosis. In this thesis, a localisation study of Rca1 was performed revealing that a nuclear localisation sequence (NLS) and other domains in the protein mediate efficient nuclear accumulation. Besides its function in G2, Rca1 expression can promote S phase entry. Rca1 belongs to the family of F-box proteins that mediate substrate specificity in SCF (Skp-Cul1-F-box) ubiquitin ligases. Functional studies demonstrated...
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free text keywords: ddc:570
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146 references, page 1 of 10

3.4.1 Fzr, Rux or Wee are not targets of the SCF/Rca1 ................................................... 39 3.4.2 Peptide Mass Fingerprinting analysis (PMF) .......................................................... 42 3.5 Rca1 function during endoreplication ..................................................................... 44 3.5.1 Gene expression analysis of endoreplicating cells .................................................. 45 3.5.2 HA-Rca1 overexpression increases Cyclin E, Rnr2 and PCNA transcript levels in endoreplicating cells ................................................................................................ 47 4.

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