publication . Article . 1983

Reverse-Phase High-Performance Liquid Chromatographic Separation of Methylated and Non-Methylated Nucleic Acid Bases

Madyastha, Prema; Rao, Pratima; Deobagkar, DN; Madyastha, KM;
Open Access
  • Published: 01 Jun 1983
  • Publisher: Indian Academy of Sciences
  • Country: India
Abstract
A high-performance liquid chromatographic sepration method is described for the detection of 5-methylcytosine and 6-methyladenine in nucleic acid ext. The bases were sepd. on a Waters $C18 \mu$ Bondapak column with a water: methanol acetic acid system. Effluents were monitored by UV absorption at 254 nm. The bases were estd. by peak heights which are proportional to the amts. of the individual bases. The method is rapid, sensitive, easy to perform and reproducible.
Subjects
free text keywords: Organic Chemistry
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