Reverse-Phase High-Performance Liquid Chromatographic Separation of Methylated and Non-Methylated Nucleic Acid Bases

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Madyastha, Prema; Rao, Pratima; Deobagkar, DN; Madyastha, KM;
  • Publisher: Indian Academy of Sciences
  • Subject: Organic Chemistry

A high-performance liquid chromatographic sepration method is described for the detection of 5-methylcytosine and 6-methyladenine in nucleic acid ext. The bases were sepd. on a Waters $C18 \mu$ Bondapak column with a water: methanol acetic acid system. Effluents were mo... View more
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