publication . Part of book or chapter of book . Article . 2006

Differentiation of Mouse Embryonic Stem Cells Into Endothelial Cells

Clotilde Gimond;
Open Access
  • Published: 01 Jan 2006
  • Publisher: Humana Press
  • Country: France
Abstract
International audience; Embryonic stem (ES) cells are pluripotent cells derived from blastocyst-stage embryos. They are characterized by their infinite self-renewal capacity and their ability to differentiate into many cell types in vitro as well as in vivo. The present protocol describes culture conditions that allow efficient differentiation of mouse ES cells toward the endothelial lineage, both in two-dimensional cultures and in three-dimensional, multicellular embryoid bodies. We also provide a protocol for establishing recombinant ES cell clones, giving the example of cells expressing green fluorescent protein and an antibiotic resistance gene under the con...
Subjects
free text keywords: MESH : Animals, MESH : Antigens, CD31, MESH : Humans, MESH : Mice, MESH : Mice, Nude, MESH : Reverse Transcriptase Polymerase Chain Reaction, MESH : Stem Cells, MESH : Cell Culture Techniques, MESH : Cell Differentiation, MESH : Cell Transplantation, MESH : Cells, Cultured, MESH : Embryo, Mammalian, MESH : Endothelial Cells, MESH : Extracellular Matrix, MESH : Green Fluorescent Proteins, [ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, MESH: Animals, MESH: Antigens, CD31, MESH: Humans, MESH: Mice, MESH: Mice, Nude, MESH: Reverse Transcriptase Polymerase Chain Reaction, MESH: Stem Cells, MESH: Cell Culture Techniques, MESH: Cell Differentiation, MESH: Cell Transplantation, MESH: Cells, Cultured, MESH: Embryo, Mammalian, MESH: Endothelial Cells, MESH: Extracellular Matrix, MESH: Green Fluorescent Proteins, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.CAN]Life Sciences [q-bio]/Cancer
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