publication . Thesis . 1989

Studies on direct and indirect electrochemical immunoassays

Buckley, Eileen;
Open Access English
  • Published: 01 Jan 1989
  • Publisher: Dublin City University. School of Chemical Sciences
  • Country: Ireland
Abstract
Two approaches to electrochemical immunoassay are reported. The first approach was an indirect method, involving an electroactive, enzyme-catalysed, substrate to product reaction. Conditions were optimised for the amperometric detection of para-aminophenol, the electroactive product of the alkaline phosphatase catalysed hydrolysis of a new substrate, p-aminophenylphosphate, after separation by HPLC. The second approach involved the direct electrochemical detection of an immunoglobulin species, IgG, and the electrochemical monitoring of the immunological interaction. In an attempt to characterise the electrochemical response of such proteins, studies were carried...
Subjects
free text keywords: Chemistry, Electrochemical analysis; Electrochemical immunoassay
38 references, page 1 of 3

2.1 Voltammetric Methods

2.1.1. Introduction

2.1.2. Basic Features of the Electrochemical Process

2.1.2.1. Mass Transfer

2.1.2.2. The Electrode Process

2.1.2.3. Faradaic and Capacitance Currents

2 .1„2 .4, The Doub1e Layer

2.1.2.4.1. Adsorption Phenomena

2.1.3. Electroanalytical Techniques

2.1.3.1. Cyclic Voltammetry

2.1.4. Pulse Techniques

2.1.4.1. Normal Pulse Voltammetry

2.1.4.2. Differential Pulse Voltammetry

2.1.5. Stripping Analysis

2.1.5.1 Introduction

38 references, page 1 of 3
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