Green fluorescent protein
Green fluorescent protein
Methods of distinguishing between fluorescent light emitted by green fluorescent protein and fluorescent light emitted by other autofluorescent mols. are described which entail using plane-polarized light to illuminate a sample contg. green fluorescent protein and auto-fluorescent mols.; detecting the intensity of fluorescent light that is emitted with a first polarization from the sample; detecting the intensity of fluorescent light that is emitted with a second polarization from the sample; subtracting a first of the detected intensities from a second of the detected intensities to obtain a difference signal; and using the difference signal to distinguish between fluorescent light emitted by green fluorescent protein and fluorescent light emitted by other fluorescent mols. in the sample. The green fluorescent protein may be expressed in response to DNA damage. [on SciFinder (R)]
- University of Salford United Kingdom
Fluorescence (autofluorescence, green fluorescent protein fluorescence discrimination endogenous autofluorescence, discriminating between fluorescence from green fluorescent protein from fluorescence from other sources), DNA damage Role: ANT (Analyte), ANST (Analytical study) (detecting and distinguishing fluorescence from green fluorescent protein expressed in response to), green fluorescent protein as), Reporter gene Role: ARU (Analytical role, unclassified), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (for DNA damage, Fluorometry, Proteins Role: ARG (Analytical reagent use), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study), USES (Uses) (green fluorescent, Polarized fluorescence (distinguishing green fluorescent protein fluorescence from endogenous autofluorescence), distinguishing green fluorescent protein fluorescence from endogenous autofluorescence)
Fluorescence (autofluorescence, green fluorescent protein fluorescence discrimination endogenous autofluorescence, discriminating between fluorescence from green fluorescent protein from fluorescence from other sources), DNA damage Role: ANT (Analyte), ANST (Analytical study) (detecting and distinguishing fluorescence from green fluorescent protein expressed in response to), green fluorescent protein as), Reporter gene Role: ARU (Analytical role, unclassified), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (for DNA damage, Fluorometry, Proteins Role: ARG (Analytical reagent use), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study), USES (Uses) (green fluorescent, Polarized fluorescence (distinguishing green fluorescent protein fluorescence from endogenous autofluorescence), distinguishing green fluorescent protein fluorescence from endogenous autofluorescence)
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