publication . Article . 2015

Comparison of colorimetric methods for the quantification of model proteins in aqueous two-phase systems.

Anna Glyk; Sandra L. Heinisch; Thomas Scheper; Sascha Beutel;
Open Access English
  • Published: 01 Jan 2015
  • Publisher: Cambridge, MA : Elsevier Academic Press
  • Country: Germany
Abstract
Abstract In the current study, the quantification of different model proteins in the presence of typical aqueous two-phase system components was investigated by using the Bradford and bicinchoninic acid (BCA) assays. Each phase-forming component above 1 and 5 wt% had considerable effects on the protein quantification in both assays, respectively, resulting in diminished protein recoveries/absorption values by increasing poly(ethylene glycol) (PEG)/salt concentration and PEG molecular weight. Therefore, a convenient dilution of both components (up to 1 and 5 wt%) before protein quantification is recommended in both assays, respectively, where the BCA assay is fav...
Subjects
free text keywords: Aqueous two-phase system, BCA assay, Bradford assay, Colorimetric analysis, Protein quantification, citrate sodium, citric acid, dextran, drug analysis, macrogol, macrogol derivative, phosphate, potassium derivative, potassium dihydrogen phosphate, water, alkalinity, bicinchoninic acid assay, chemical phenomena, chemistry, colorimetry, comparative study, intermethod comparison, limit of quantitation, molecular weight, priority journal, procedures, protein binding, protein determination, viscosity, analysis, Citrates, Phosphates, Polyethylene Glycols, Potassium Compounds, Proteins, ddc:540, ddc:570, ddc:500, Chromatography, Ethylene glycol, chemistry.chemical_compound, Bradford protein assay, Biochemistry, Detection limit, PEG ratio
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publication . Article . 2015

Comparison of colorimetric methods for the quantification of model proteins in aqueous two-phase systems.

Anna Glyk; Sandra L. Heinisch; Thomas Scheper; Sascha Beutel;