Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA

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Vergara, Mauricio; Becerra, Silvana; Diaz-Barrera, Alvaro; Berrios, Julio; Altamirano, Claudia;
  • Publisher: Pontificia Universidad Católica de Valparaíso
  • Related identifiers: doi: 10.2225/vol15-issue6-fulltext-2
  • Subject: perfusion culture | rh-tPA | CHO cells | galactose | low temperature | glutamate

We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activ... View more
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