Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA

Article English OPEN
Vergara,Mauricio; Becerra,Silvana; Díaz-Barrera,Alvaro; Berrios,Julio; Altamirano,Claudia;
  • Publisher: Pontificia Universidad Católica de Valparaíso
  • Related identifiers: doi: 10.2225/vol15-issue6-fulltext-2
  • Subject: perfusion culture | rh-tPA | CHO cells | galactose | low temperature | glutamate

We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activ... View more
  • References (34)
    34 references, page 1 of 4

    AL-FAGEEH, M.; MARCHANT, R.; CARDEN, M. and SMALES, C. (2006). The cold-shock response in cultured mammalian cells: Harnessing the response for the improvement of recombinant protein production. Biotechnology and Bioengineering, vol. 93, no. 5, p. 829-835. [CrossRef]

    ALTAMIRANO, C.; PAREDES, C.; CAIRÓ, J. and GÒDIA, F. (2000). Improvement of CHO cell culture medium formulation: Simultaneous substitution of glucose and glutamine. Biotechnology Progress, vol. 16, no. 1, p. 69-75. [CrossRef]

    ALTAMIRANO, C.; CAIRÓ, J. and GÒDIA, F. (2001). Decoupling cell growth and product formation in Chinese hamster ovary cells through metabolic control. Biotechnology and Bioengineering, vol. 76, no. 4, p. 351-360. [CrossRef]

    ALTAMIRANO, C.; ILLANES, A.; CANESSA, R. and BECERRA, S. (2006). Specific nutrient supplementation of defined serum-free medium for the improvement of CHO cells growth and t-PA production. Electronic Journal of Biotechnology, vol. 9, no. 1, p. 61-67. [CrossRef]

    BAIK, J.K.; LEE, M.S.; AN, S.R.; YOON, S.K.; JOO, E.J.; KIM, Y.H.; PARK, H.W. and LEE, G.M. (2006). Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin. Biotechnology and Bioengineering, vol. 93, no. 2, p. 361-371. [CrossRef]

    BARNES, L. and DICKSON, A. (2006). Mammalian cell factories for efficient and stable protein expression. Current Opinion in Biotechnology, vol. 17, no. 4, p. 381-386. [CrossRef]

    BECERRA, S.; BERRIOS, J.; OSSES, N. and ALTAMIRANO, C. (2012). Exploring the effect of mild hypothermia on CHO cell productivity. Biochemical Engineering Journal, vol. 60, p. 1-8. [CrossRef]

    BERRIOS, J.; DÍAZ-BARRERA, A.; BAZÁN, C. and ALTAMIRANO, C. (2009). Relationship between tissue plasminogen activator production and specific growth rate in Chinese hamster ovary cells cultured in mannose at low temperature. Biotechnology Letters, vol. 31, no. 10, p. 1493-1497. [CrossRef]

    BERRIOS, J.; ALTAMIRANO C.; OSSES, N. and GONZALEZ, R. (2011). Continuous CHO cell cultures with improved recombinant protein productivity by using mannose as carbon source: Metabolic analysis and scale-up simulation. Chemical Engineering Science, vol. 66, no. 11, p. 2431-2439. [CrossRef]

    BI, J.; SHUTTLEWORTH, J. and AL-RUBEAI, M. (2004). Uncoupling of cell growth and proliferation results in enhancement of productivity in p21Cip1-arrested CHO cells. Biotechnology and Bioengineering, vol. 85, no. 7, p. 741-749. [CrossRef]

  • Metrics