publication . Article . 1997

A comparison of in vitro methods for assessing the potency of therapeutic antisera against the venom of the coral snake Micrurus nigrocinctus.

Alape Girón, Alberto; Miranda Arrieta, Keyna; Cortés Bratti, Ximena; Stiles, Bradley G.; Gutiérrez, José María;
Open Access
  • Published: 01 Apr 1997 Journal: Toxicon, volume 35, pages 573-581 (issn: 0041-0101, Copyright policy)
  • Publisher: Elsevier BV
Abstract
Therapeutic antisera against Micrurus nigrocinctus venom were tested for protection against lethality, as well as for inhibition of the nicotinic acetylcholine receptor (AchR)-binding and neutralization of phospholipase A2 (PLA2) activities of the homologous venom. Protection against venom lethality did not correlate with inhibition of AchR-binding activity, whereas there was a significant correlation between antisera potency and inhibition of PLA2 activity (r = 0.82, n = 10, P < 0.02). Inhibition of PLA2 activity could be useful in assessing the protective efficacy of M. nigrocinctus antisera during antivenom production. Micrurus nigrocinctus nigrocinctus venom...
Subjects
Medical Subject Headings: complex mixtures
free text keywords: Animals, Antivenins, Elapidae, Enzyme-Linked Immunosorbent Assay, Mice, Phospholipases A, Phospholipases A2, Receptors, Nicotinic, Snake Bites, Toxicology, Venom, Elapid Venoms, Micrurus nigrocinctus, biology.organism_classification, biology, Fast protein liquid chromatography, Coral snake, Biochemistry, Snake venom, Potency, Antivenom
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publication . Article . 1997

A comparison of in vitro methods for assessing the potency of therapeutic antisera against the venom of the coral snake Micrurus nigrocinctus.

Alape Girón, Alberto; Miranda Arrieta, Keyna; Cortés Bratti, Ximena; Stiles, Bradley G.; Gutiérrez, José María;