Liquor oligoclonal bands assay: interpretation, correlation with other laboratory assays and importance for diagnostics of neurological disorders

Master thesis Lithuanian OPEN
Bagdonas, Dovydas (2017)
  • Publisher: Institutional Repository of Lithuanian University of Health Sciences
  • Subject: IgG oligoclonal bands assay ; liquor biochemical assays ; blood serum biochemical assays ; multiple sclerosis ; demyelinating encephalomyelitis
    mesheuropmc: food and beverages | equipment and supplies

Aim: to analyse the possible relationship between liquor IgG oligoclonal bands assay and other laboratory assays in neurological patients. Objectives: to determine the frequency of oligoclonal bands in neurological patients; to compare the results between serum and liquor laboratory assays in dependence of oligoclonal bands assay results; to evaluate the relationships between oligoclonal bands assay and serological-immunological assays for infectious diseases, gender, age and neurological diseases. Methods. 2 year retrospective analysis of medical documentation from 160 patients after IgG oligoclonal bands assay from cerebrospinal fluid was performed in LSMUL KK. Collected clinical, demographic and laboratory assay data was analysed using statistical methods. Results. Analysed patient age average was 41,94 years, 59% were diagnosed with multiple sclerosis, 34% with demyelinating encephalomyelitis, the rest – with other neurological diseases. Patients with multiple sclerosis had liquor oligoclonal bands most frequently, had highest serum IgG concentration in comparison with all analysed diseases, their liquor IgG concentration and IgG index were higher than norm and than patients suffering from demyelinating encephalomyelitis. Patients with liquor oligoclonal bands had elevated liquor IgG concentration, IgG index, higher liquor leucocyte count and lower liquor glucose concentration (p<0,05) than patients without liquor oligoclonal bands. Blood serum creatinine and IgG concentrations were higher in group with positive liquor oligoclonal bands assay result when compared to the group with negative oligoclonal bands assay result. Statistically significantly (p<0,05) higher liquor total protein and albumin as well as serum albumin, creatinine, urea concentrations were discovered in males; also, older people had lower protein and higher metabolite concentrations. Conclusions. Liquor oligoclonal bands were most frequent in multiple sclerosis sufferers. Neurological patients had higher liquor IgG, IgG index and leucocyte count values and lower glucose concentrations as well as higher protein metabolite and IgG concentrations in blood serum when liquor oligoclonal bands assay was positive, especially in elderly men. Oligoclonal bands in liquor have a relationship with multiple sclerosis and demyelinating encephalomyelitis but no relations with gender, age and the markers of infectious diseases.
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