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Диагностическая значимость серологических и молекулярно-генетических методов для выявления краснушной инфекции

Диагностическая значимость серологических и молекулярно-генетических методов для выявления краснушной инфекции

Abstract

The early diagnostic of German measles infection, especially in cases requiring differential diagnostic search, the most informative and usable in practice test-systems are needed to be applied. The sampling of patients (n=37) included males aged from 15 to 21 year (average age 18 years) were admitted to hospital on 1-3 day from onset of disease. The technique of polymerase chain reaction with reverse transcription was applied to detect presence of viral RNA in nasopharyngital smear. The presence of viral RNA was confirmed in 26 examined patients (70%). The serological markers of onset of disease at the moment of first examination had 24 (65%) out of 37 patients. It is demonstrated that technique of polymerase chain reaction with reverse transcription has the most diagnostic value in confirmation of diagnosis of German measles infection at the first days of disease. In the sequel, the informativeness of methods of serological diagnostic will increase because complex application of methods of IgM and polymerase chain reaction with reverse transcription are needed at different periods of disease.

Для ранней диагностики краснушной инфекции, особенно в случаях, требующих дифференциально-диагностического поиска, крайне важно использовать наиболее информативные и удобные в практической работе тест-системы. Пациенты (n = 37), мужчины в возрасте от 15 лет до 21 года (средний возраст 18 лет), поступали в стационар на 1-3-й день от начала заболевания. Наличие вирусной РНК в назофарингеальных мазках методом ОТ-ПЦР (ПЦР с обратной транскрипцией) было подтверждено у 26 (70%) обследованных, серологические маркеры начала краснушной инфекции на момент первого обследования имели 24 (65%) больных из 37 обследованных. Показано, что наибольшую диагностическую значимость в подтверждении диагноза краснушной инфекции в первые дни заболевания имеет метод ОТ-ПЦР, в дальнейшем возрастает информативность методов серодиагностики, на разных сроках заболевания требуется комплексное применение методов ИФА (IgM) и ОТ-ПЦР.

Keywords

ДИАГНОСТИКА КРАСНУХИ, ПОЛИМЕРАЗНАЯ ЦЕПНАЯ РЕАКЦИЯ С ОБРАТНОЙ ТРАНСКРИПЦИЕЙ (ОТ-ПЦР), СЕРОЛОГИЧЕСКИЕ МАРКЕРЫ

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
gold