Characterization and evaluation of some lines from cherry tomatoes lycopersicon esculentum mill. Var. Cerasiforme (dunal) and their ability for micropropagation in in vitro conditions

Doctoral thesis OPEN
Dedejski, George (2012)
  • Subject: Agricultural biotechnology

Tomato production in the Republic of Macedonia is present on more than 5700 hectares, being the leading vegetable crop in the region of Strumica. Cherry tomato however, is poorly present at our fields, mainly due to the traditional habits of the consumers and the commercial tomato producers to grow tomato varieties with large fruit. Cherry tomato - Lycopersicon esculentum Mill. var. cerasiforme (Dunal) is a tomato variety with small fruit, with different shapes and colors and it is used mainly for fresh consumption. The characteristic of this variety are its sweetness and aroma, which further enriches the taste of food. During this research, a comparative analysis of the morphological traits in this type of tomato in outdoor production conditions and in protected environment was done. The possibilities for production and maintenance in plant cell cultutre were researched as well, with the goal of future improving the morphological and biological features of cherry tomato. The characterization and comparative evaluation of the genotypes was done according to the modified descriptors for tomato of Bioversity International (formerly International Plant Genetic Resources Institute-IPGRI). For each genotype, the morphological characteristics of the plants and fruits were measured in three distinctive stages: planting phase, flowering phase and the stage of botanical maturity of the fruits. Genotypes from cherry tomato were placed in culture in vitro in order to evaluate the effect of different tomato genotypes of cherry tomatoes, and different growth regulators on callus formation and their ability to regenerate. All results obtained during this research were statistically processed and analyzed with statistical software IBM SPSS Statistics 19. The mean values obtained for the different studied parameters were compared with One-way ANOVA (Duncan posthoc) test with significance level of 0,05%.
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