publication . Article . Other literature type . 2008

A microscale method for colorimetric determination of urea in soil extracts

Greenan, N.S.; Mulvaney, R.L.; Sims, G. K.;
Open Access
  • Published: 11 Nov 2008 Journal: Communications in Soil Science and Plant Analysis, volume 26, pages 2,519-2,529 (issn: 0010-3624, eissn: 1532-2416, Copyright policy)
  • Publisher: Informa UK Limited
Abstract
Abstract The diacetyl monoxime colorimetric method of determining urea in soil extracts was modified for microplate format. A 100‐μL aliquot of extract was treated with color reagent in a disposable plastic microtiter plate (96 wells/plate), and color was developed by heating the plate in a low‐temperature oven at 87°C for 55 min. After cooling for 20 min at ambient temperature, absorbance measurements were simultaneously performed on all 96 wells using a microplate reader. The microscale method was faster and more convenient than the conventional method; moreover, the volume of waste was markedly reduced. Studies to compare the two methods showed very little di...
Subjects
free text keywords: Agronomy and Crop Science, Soil Science, Chemistry, Reagent, Urea, chemistry.chemical_compound, Microplate Reader, Colorimetry, Absorbance, Colorimetry (chemical method), Microtiter plate, Microscale chemistry, Analytical chemistry
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publication . Article . Other literature type . 2008

A microscale method for colorimetric determination of urea in soil extracts

Greenan, N.S.; Mulvaney, R.L.; Sims, G. K.;