publication . Article . 1987

Production of Human Tissue Plasminogen Activator in Transgenic Mouse Milk

Katherine Gordon; Eric Lee; James A. Vitale; Alan E. Smith; Heiner Westphal; Lothar Hennighausen;
Open Access
  • Published: 01 Nov 1987
We set out to express an exogenous gene in the mammary epithelium of transgenic mice in the hope that the encoded protein would be secreted into milk. The promoter and upstream regulatory sequences from the murine whey acid protein (WAP) gene were fused to cDNA encoding human tissue plasminogen activator (t–PA) with its endogenous secretion signal sequence. This hybrid gene was injected into mouse embryos, resultant transgenic mice were mated, and milk obtained from lactating females was shown to contain biologically active t–PA. This result establishes the feasibility of secretion into the milk of transgenic animals for production of biologically active heterol...
free text keywords: Genetically modified mouse, Plasminogen activator, Regulatory sequence, Complementary DNA, Molecular biology, Heterologous, Secretion, Signal peptide, Transgene, Biology
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