publication . Article . 1999

Bacterial promoter architecture: subsite structure of UP elements and interactions with the carboxy-terminal domain of the RNA polymerase alpha subunit

Richard L. Gourse; Z. W. S. Chen; Wei Niu; Tamas Gaal; Richard H. Ebright; Wilma Ross; Shawn T. Estrem;
Open Access
  • Published: 15 Aug 1999
Abstract
We demonstrate here that the previously described bacterial promoter upstream element (UP element) consists of two distinct subsites, each of which, by itself, can bind the RNA polymerase holoenzyme a subunit carboxy-terminal domain (RNAP aCTD) and stimulate transcription. Using binding-site-selection experiments, we identify the consensus sequence for each subsite. The selected proximal subsites (positions ˛46 to ˛38; consensus 5*-AAAAAARNR-3*) stimulate transcription up to 170-fold, and the selected distal subsites (positions ˛57 to ˛47; consensus 5*-AWWWWWTTTTT-3*) stimulate transcription up to 16-fold. RNAP has subunit composition a2bb*s and thus contains tw...
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free text keywords: Developmental Biology, Genetics, RNA polymerase II holoenzyme, Α subunit, Biology, Molecular biology, Binding site, Consensus sequence, Transcription (biology), RNA polymerase, chemistry.chemical_compound, chemistry, Protein subunit
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