publication . Article . Other literature type . 2014

MERS-CoV PCR/Sequencing Primers

Sprotocols;
Open Access
  • Published: 31 Dec 2014
Abstract
Authors: Rachel Graham ### Abstract This protocol details the primers and conditions used for forward and reverse PCR amplification and sequencing of MERS-CoV genomes. ### Introduction This protocol details the steps, reagents, and conditions required to sequence MERS-CoV genomes in the forward and reverse directions. The protocol begins with the RT-PCR step, assuming that MERS-CoV RNA purified using a standard procedure (i.e., TRIzol extraction) has already been performed. ### Materials 1. Invitrogen SuperScript III kit - dNTPS (10 mM) - Random Hexamers - RNasin (if desired) - Thermo Phusion PCR enzyme - Primers (2 mM stock – see Tables 1 and 2) - Agarose - 1X ...
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Article . 2014
Provider: Datacite
Zenodo
Other literature type . 2014
Provider: Datacite
ZENODO
Article . 2014
Provider: ZENODO
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