publication . Other literature type . Article . 2015

Cell Volume And Geometric Parameters Determination In Living Cells Using Confocal Microscopy And 3D Reconstruction

David Hevia; David Hevia; Aida Rodriguez-Garcia; Marta Alonso-Gervós; Isabel Quirós-González; Henar M Cimadevilla; Carmen Gómez-Cordovés; Rosa M Sainz; Juan C Mayo;
Open Access
  • Published: 18 Jan 2015
  • Publisher: Zenodo
Abstract
Authors: David Hevia, Aida Rodriguez-Garcia, Marta Alonso-Gervós, Isabel Quirós-González, Henar M Cimadevilla, Carmen Gómez-Cordovés, Rosa M Sainz & Juan C Mayo ### Abstract The protocol reported here describes a simple, easy, fast and reproducible method aimed to know the geometric parameters of living cells based on confocal laser scanning microscopy combined with 3D reconstruction software. Briefly, the method is based on intrinsic fluorescence properties of acridine orange (AO), a molecule taken up by living adherent cells. Dual binding of AO to either DNA or RNA allows complete staining. When combined with confocal microscopy, 3D software can be used fo...
Subjects
free text keywords: Chemistry, Confocal microscopy, law.invention, law, Cell volume, Biomedical engineering, 3D reconstruction
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Other literature type . 2015
Provider: Datacite
ZENODO
Article . 2015
Provider: ZENODO
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publication . Other literature type . Article . 2015

Cell Volume And Geometric Parameters Determination In Living Cells Using Confocal Microscopy And 3D Reconstruction

David Hevia; David Hevia; Aida Rodriguez-Garcia; Marta Alonso-Gervós; Isabel Quirós-González; Henar M Cimadevilla; Carmen Gómez-Cordovés; Rosa M Sainz; Juan C Mayo;