publication . Article . 2018

A novel specific duplex real-time RT-PCR method for absolute quantitation of Grapevine Pinot gris virus in plant material and single mites

Félix Morán; Antonio Olmos; Leonidas Lotos; Lukáš Predajňa; Nikolaos I. Katis; Miroslav Glasa; Varvara I. Maliogka; Ana Belén Ruiz-García;
Open Access
  • Published: 15 May 2018 Journal: PLOS ONE, volume 13, page e0197237 (eissn: 1932-6203, Copyright policy)
  • Publisher: Public Library of Science (PLoS)
Abstract
Grapevine Pinot gris virus (GPGV) is a widely distributed grapevine pathogen that has been associated to the grapevine leaf mottling and deformation disease. With the aim of better understanding the disease epidemiology and providing efficient control strategies a specific and quantitative duplex TaqMan real-time RT-PCR assay has been developed. This method has allowed reliable quantitation of the GPGV titer ranging from 30 up to 3 x 108 transcript copies, with a detection limit of 70 viral copies in plant material. The assay targets a grapevine internal control that reduces the occurrence of false negative results, thus increasing the diagnostic sensitivity of ...
Persistent Identifiers
Subjects
free text keywords: General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, General Medicine, Research Article, Biology and Life Sciences, Organisms, Eukaryota, Plants, Flowering Plants, Grapevine, Molecular Biology, Molecular Biology Techniques, Artificial Gene Amplification and Extension, Polymerase Chain Reaction, Reverse Transcriptase-Polymerase Chain Reaction, Research and Analysis Methods, Medicine and Health Sciences, Infectious Diseases, Infectious Disease Control, Animals, Invertebrates, Arthropoda, Mites, Evolutionary Biology, Evolutionary Systematics, Phylogenetics, Taxonomy, Computer and Information Sciences, Data Management, Database and Informatics Methods, Bioinformatics, Sequence Analysis, Sequence Alignment, Physical Sciences, Physics, Classical Mechanics, Deformation, Damage Mechanics, Genetics, Genomics, GPGV, Grapevine, Real-time RT-PCR, Quantification, Virology, TaqMan, Genome, Movement protein, Titer, Trichovirus, biology.organism_classification, biology, Polymerase chain reaction, law.invention, law, Flexiviridae, Genomics, lcsh:Medicine, lcsh:R, lcsh:Science, lcsh:Q
Funded by
EC| VirFree
Project
VirFree
Virus free Fruit Nurseries
  • Funder: European Commission (EC)
  • Project Code: 734736
  • Funding stream: H2020 | MSCA-RISE
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