publication . Article . Other literature type . 2015

The electrophoretic mobility shift assay (EMSA)

Erika Lackey; Z. Jeffrey Chen;
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  • Published: 13 Apr 2015 Journal: Protocol Exchange (eissn: 2043-0116, Copyright policy)
  • Publisher: Springer Science and Business Media LLC
Abstract
The electrophoretic mobility shift assay (EMSA), also known as “gel shift assay”, is used to examine the binding parameters and relative affinities of protein and DNA interactions. We produced recombinant CCA1 protein and tested its binding affinity for the promoter fragments that contain CBS (AAAAATCT) or evening element (EE, AAAATATCT) (1) using a modified procedure adopted from published protocols (2,3).
Subjects
free text keywords: Biochemistry, Molecular biology, Biology, Electrophoretic mobility shift assay
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Other literature type . 2015
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publication . Article . Other literature type . 2015

The electrophoretic mobility shift assay (EMSA)

Erika Lackey; Z. Jeffrey Chen;