publication . Article . 2009

Detection of Plasmodium sp. in capybara.

dos Santos Leonilda Correia; Curotto Sandra Mara Rotter; de Moraes Wanderlei; Cubas Zalmir Silvino; Costa-Nascimento Maria de Jesus; Filho Ivan Roque de Barros; Biondo Alexander Welker; Kirchgatter Karin;
Open Access
  • Published: 01 Jul 2009
Abstract
Abstract In the present study, we have microscopically and molecularly surveyed blood samples from 11 captive capybaras (Hydrochaeris hydrochaeris) from the Sanctuary Zoo for Plasmodium sp. infection. One animal presented positive on blood smear by light microscopy. Polymerase chain reaction was carried out accordingly using a nested genus-specific protocol, which uses oligonucleotides from conserved sequences flanking a variable sequence region in the small subunit ribosomal RNA (ssrRNA) of all Plasmodium organisms. This revealed three positive animals. Products from two samples were purified and sequenced. The results showed less than 1% divergence between the...
Subjects
Medical Subject Headings: parasitic diseases
free text keywords: General Veterinary, Parasitology, Plasmodium mexicanum, biology.organism_classification, biology, GenBank, Ribosomal RNA, Conserved sequence, Polymerase chain reaction, law.invention, law, RNA, Toxoplasma gondii, Virology
Powered by OpenAIRE Open Research Graph
Any information missing or wrong?Report an Issue
publication . Article . 2009

Detection of Plasmodium sp. in capybara.

dos Santos Leonilda Correia; Curotto Sandra Mara Rotter; de Moraes Wanderlei; Cubas Zalmir Silvino; Costa-Nascimento Maria de Jesus; Filho Ivan Roque de Barros; Biondo Alexander Welker; Kirchgatter Karin;