publication . Article . 2009

Human hydroxyindole-O-methyltransferase: presence of LINE-1 fragment in a cDNA clone and pineal mRNA.

Susan J. Donohue; Patrick H. Roseboom; Helena Illnerova; Joan L. Weller; David C. Klein;
Open Access
  • Published: 24 Mar 2009
  • Publisher: DNA and cell biology
Abstract
None: Hydroxyindole-O-methyltransferase (HIOMT) catalyzes the last step in the synthesis of the pineal hormone melatonin. In this study, an HIOMT clone was isolated from a human pineal cDNA library using synthetic oligonucleotide probes based on the bovine HIOMT sequence. The human sequence is unusual because it contains a 3' fragment (84 bp) of the LINE-1 sequence, a highly repetitive sequence in the human genome and the genome of some primates and rodents. Exclusive of this LINE-1 fragment, the human HIOMT clone is 75% and 63% homologous to bovine and avian HIOMT sequences, respectively. The deduced amino acid sequence of the human cDNA clone encodes a 41.6-kD...
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free text keywords: Genetics, Cell Biology, Molecular Biology, General Medicine, Oligonucleotide, Methyltransferase, Amino acid, chemistry.chemical_classification, chemistry, Human genome, Peptide sequence, Biology, Messenger RNA, cDNA library, clone (Java method), Molecular biology
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