publication . Other literature type . Article . 2019

Luciferase-Induced Photouncaging: Bioluminolysis

Nicolas Winssinger; Eric Lindberg; Eric Lindberg; Howard Riezman; Dalu Chang; Simona Angerani; Suihan Feng;
Open Access
  • Published: 22 Aug 2019
  • Publisher: Zenodo
  • Country: Switzerland
Abstract
Bioluminescence resonance energy transfer (BRET) has been widely used for studying dynamic processes in biological systems such as protein-protein interactions and other signaling events. Aside from acting as a reporter, BRET can also turn on functions in living systems. Herein, we report the application of BRET to performing a biorthogonal reaction in living cells; namely, releasing functional molecules through energy transfer to a coumarin molecule, a process termed bioluminolysis. An efficient BRET from Nanoluc-Halotag chimera protein (H-Luc) to a coumarin substrate yields the excited state of coumarin, which in turn triggers hydrolysis to uncage a target mol...
Subjects
free text keywords: General Chemistry, Catalysis, Chemistry, Bioluminescence, Luciferase, Kinetics, In vivo, Kinase, Biophysics, Coumarin, chemistry.chemical_compound, Small molecule, Molecule, ddc:540
Related Organizations
Funded by
SNSF| Probes for chemical biology: from synthesis to biosupramolecular systems
Project
  • Funder: Swiss National Science Foundation (SNSF)
  • Project Code: 200020_169141
  • Funding stream: Project funding | Project funding (Div. I-III)
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Zenodo
Other literature type . 2019
Provider: Datacite
ZENODO
Article . 2019
Provider: ZENODO
Zenodo
Other literature type . 2019
Provider: Datacite
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