publication . Article . 1990

Differential effects of N-methyl-N′-nitro-N-nitrosoguanidine on constitutive and hormone-inducible gene expression in rat hepatoma cells

Mark Steven Miller;
Open Access
  • Published: 01 Jan 1990
Abstract Previous studies have shown that treatment of rat hepatoma cells (the Fao clone of Reuber H-35 cells) with 500 ng/ml of N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) causes a 78% decrease in dexamethasone (DEX)-induced tyrosine aminotransferase (TAT) enzyme activity and a concurrent 75% decline in total steroid-induced TAT steady-state RNA levels. To determine if this inhibition was a specific or more general effect on inducible-gene expression, the effects of MNNG on other genes were examined. MNNG had little effect on total DEX or Cd-induced metallothionein (MT) RNA levels when the cells were treated with 1 μM DEX or 3 μM CdCl2 for 4 h. In addition, the...
free text keywords: Chemistry, Biochemistry, Molecular biology, Gene expression, Liver function, Gene, RNA, Methylnitronitrosoguanidine, chemistry.chemical_compound, Glucocorticoid receptor, Tyrosine aminotransferase, Transcription (biology)
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