publication . Article . Other literature type . 2015

Monitoring protein synthesis by fluorescence recovery after photobleaching (FRAP) in vivo

Sprotocols;
Open Access
  • Published: 11 May 2015
Abstract
Currently available methodologies for measuring protein synthesis rates rely on metabolic labelling by incorporation of radioactive amino acids into nascent polypeptides. These approaches are hampered by several limitations and cannot be applied to monitor protein synthesis in specific cells or tissues, in live specimens. Here, we describe a novel method for monitoring protein synthesis in specific cells and tissues of live Caenorhabditis elegans animals. Fluorescent reporter proteins such as GFP are expressed in specific cells and tissues of interest or throughout animals using appropriate promoters. Protein synthesis rates are assessed by following fluorescenc...
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ZENODO
Article . 2015
Provider: ZENODO
Zenodo
Other literature type . 2015
Provider: Datacite
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