publication . Other literature type . Article . 2011

Two dimensional gel electrophoresis using narrow pH 3-5.6 immobilised pH gradient strips identifies potential novel disease biomarkers in plasma or serum

Bevin Gangadharan; Bevin Gangadharan; Nicole Zitzmann;
Open Access
  • Published: 11 Oct 2011
  • Publisher: Zenodo
  • Country: United Kingdom
Abstract
Authors: Bevin Gangadharan & Nicole Zitzmann ### Abstract Two-dimensional gel electrophoresis (2-DE) is a protein separation technique often used to separate plasma or serum proteins in an attempt to identify novel biomarkers. This protocol describes how to run 2-DE gels using narrow pH 3-5.6 immobilised pH gradient strips to separate 2 mg of serum proteins. pH 3-6 ampholytes are used to enhance the solubility of proteins in this pH range before the serum proteins are separated in the first dimension by isoelectric point (isoelectric focusing) followed by molecular weight (SDS-PAGE). This approach using the pH 3-5.6 range differs from pH ranges more commonly...
Subjects
free text keywords: Isoelectric focusing, Two-dimensional gel electrophoresis, Protein purification, Isoelectric point, Solubility, Analytical chemistry, Chemistry, Chromatography, Gel electrophoresis, Blood proteins, Immobilized pH gradient
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publication . Other literature type . Article . 2011

Two dimensional gel electrophoresis using narrow pH 3-5.6 immobilised pH gradient strips identifies potential novel disease biomarkers in plasma or serum

Bevin Gangadharan; Bevin Gangadharan; Nicole Zitzmann;