publication . Article . Other literature type . 1993

Oxygen-radical absorbance capacity assay for antioxidants

Richard G. Cutler; Helaine M. Alessio; Guohua Cao;
Open Access
  • Published: 01 Mar 1993 Journal: Free Radical Biology and Medicine, volume 14, pages 303-311 (issn: 0891-5849, Copyright policy)
  • Publisher: Elsevier BV
Abstract
Abstract A relatively simple but sensitive and reliable method of quantitating the oxygen-radical absorbing capacity (ORAC) of antioxidants in serum using a few μl is described. In this assay system, β-phycoerythrin (β-PE) is used as an indicator protein, 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) as a peroxyl radical generator, and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, a water-soluble vitamin E analogue) as a control standard. Results are expressed as ORAC units, where 1 ORAC unit equals the net protection produced by 1 μM Trolox. The uniqueness of this assay is that total antioxidant capacity of a sample is estimated by taki...
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Subjects
free text keywords: Vitamin C, Chromatography, Albumin, Trolox, chemistry.chemical_compound, chemistry, Vitamin E, medicine.medical_treatment, medicine, Bovine serum albumin, biology.protein, biology, Uric acid, Oxygen radical absorbance capacity, Antioxidant, Biochemistry
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