publication . Article . 2012

Two-photon excitation with pico-second fluorescence lifetime imaging to detect nuclear association of flavanols

Juergen Polster; Anthony W. Parker; Lucie Trnková; Irene Mueller-Harvey; Walter Feucht; Pierre Burgos; Stanley W. Botchway;
Open Access
  • Published: 01 Mar 2012 Journal: Analytica Chimica Acta, volume 719, pages 68-75 (issn: 0003-2670, Copyright policy)
  • Publisher: Elsevier BV
Abstract
Two-photon excitation enabled for the first time the observation and measurement of excited state fluorescence lifetimes from three flavanols in solution, which were ∼1.0 ns for catechin and epicatechin, but <45 ps for epigallocatechin gallate (EGCG). The shorter lifetime for EGCG is in line with a lower fluorescence quantum yield of 0.003 compared to catechin (0.015) and epicatechin (0.018). In vivo experiments with onion cells demonstrated that tryptophan and quercetin, which tend to be major contributors of background fluorescence in plant cells, have sufficiently low cross sections for two-photon excitation at 630 nm and therefore do not interfere with detec...
Subjects
Medical Subject Headings: food and beverages
free text keywords: Analytical Chemistry, Spectroscopy, Biochemistry, Environmental Chemistry, Catechin, chemistry.chemical_compound, chemistry, Two-photon excitation microscopy, Epigallocatechin gallate, Tryptophan, Nucleus, medicine.anatomical_structure, medicine, Fluorescence-lifetime imaging microscopy, Quantum yield, Biophysics, Fluorescence
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