Study of the side effects of Docetaxel as chemotherapy medicine on changing the expression of genes of Enterococcus faecalis isolated from patients with breast cancer

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Farnaz Rahbarzare ; Elham Eslahi ; Nazafarin Hatami-Mazinani ; Elham Magharehabed ; Mahsa Aliqanbari ; Niloofar Ahmadi ; Shabnam Naderifar ; Mona Mirgeloybayat ; Hamed Hojatian ; Mojdeh Naeima ; Soha Sadeghi (2019)

The present study strived to ascertain the side effects of chemotherapy on the pathogenic potential of Enterococcus faecalis (a natural microflora bacteria) isolated from patients with breast cancer. Participants included 400 female sufferers from breast cancer before and after the period of chemotherapy and 400 healthy people living with patients.After RNA extracted from the stool of all the participants, cDNA was constructed. Nineteen virulent genes (vanB, vanA, VanC-3,VanC-2, VanC-1, aac(6′)-Ie-aph(2″)-Ia, Tet(L), Erm(B), gelE, esp, gyrA,slyA, cylA,cylB,cylM, asa1, aggA, efaA and enlA) of E. faecalis were assessed by Real-Time qPCR. The findings revealed a statistically significant correlation between the expression level of fourteen virulence genes(vanA(p = 0.033), vanB(p = 0.003), VanC-3(p = 0.003), aac(6′)-Ie-aph(2″)-Ia(p = 0.005), Erm(B) (p = 0.008), gelE(p = 0.002), esp(p = 0.0005), gyrA(p = 0.003), slyA(p = 0.001), cylA(p = 0.003), cylB(p = 0.0002), asa1(p = 0.003), aggA(p = 0.003), efaA(p = 0.003))in the group of the sufferers after chemotherapy and the other two groups (P<0.05).Additionally, the observation of patient group after chemotherapy showed an outstanding association between overexpression ofantibiotic resistance genes (vanA, vanB, VanC-3, Erm(B), aac(6′)-Ie-aph(2″)-Ia) and observation of the antibiotic resistance (vancomycin, erythromycin, gentamicin in respectively). Interestingly, while Docetaxel seems to be a suitable medicine to treat breast cancer, it may change the balance of mRNA expression of microflora. These could trigger infections during the cancer chemotherapy.
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