publication . Article . Other literature type . 2015

Measurement of neuron soma size by fluorescent Nissl stain

James Cronk; James Cronk; Noel Derecki; Jonathan Kipnis;
Open Access
  • Published: 10 Jan 2015 Journal: Protocol Exchange (eissn: 2043-0116, Copyright policy)
  • Publisher: Springer Nature
Abstract
Authors: James Cronk, Noel Derecki & Jonathan Kipnis ### Abstract This protocol describes how to measure neuron soma size by fluorescent Nissl stain. Mice are sacrificed, and fixed by PFA perfusion. Brains are removed, and further PFA fixed, followed by sucrose cryoprotection. They are then snap frozen, sliced by cryostat, and stained with fluorescent Nissl as floating sections. Confocal microscopy is used to take images of neurons, and a computer graphics tablet is used to calculate individual neuron soma sizes. ### Reagents 1. 5u/ml Heparin PBS - 4% PFA in PBS - 30% Sucrose in PBS - 0.1% Sodium Azide in PBS - 2-methylbutane - Dry ice - OCT Compound - 0.1% ...
Subjects
free text keywords: Fluorescence, Chemistry, Anatomy, Soma, medicine.anatomical_structure, medicine, Nissl body, symbols.namesake, symbols, Neuron
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Other literature type . 2015
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Article . 2015
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publication . Article . Other literature type . 2015

Measurement of neuron soma size by fluorescent Nissl stain

James Cronk; James Cronk; Noel Derecki; Jonathan Kipnis;