publication . Part of book or chapter of book . 2008

Quantitative Real-Time PCR: Fluorescent Probe Options and Issues

Holden, Marcia J.; Wang, Lili;
Open Access
  • Published: 12 Mar 2008
  • Publisher: Springer Berlin Heidelberg
Abstract
Fluorescence has played a vital role in the development of polymerase chain reaction (PCR)-based DNA amplification. In qualitative PCR, an end point reaction, the amplified DNA, is visualized using DNA intercalating fluorescent dyes. Creative uses of nucleotide probes with fluorescent tags have been developed for real-time quantitative PCR. These probes take advantage of the behavior and properties of fluorophores. There are advantages and disadvantages to various probe types as well as design considerations. Attention to these issues will help in the development of robust and accurate DNA quantification using real-time PCR. Open image in new window
Subjects
free text keywords: Molecular biology, DNA, chemistry.chemical_compound, chemistry, Real-time polymerase chain reaction, Polymerase chain reaction, law.invention, law, Molecular beacon, TaqMan, Hybridization probe, Förster resonance energy transfer, SYBR Green I
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Part of book or chapter of book . 2008
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publication . Part of book or chapter of book . 2008

Quantitative Real-Time PCR: Fluorescent Probe Options and Issues

Holden, Marcia J.; Wang, Lili;