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In Vivo Feedback Control of an Antithetic Molecular-Titration Motif in Escherichia coli Using Microfluidics

Shannon, Barbara; Zamora-Chimal, Criseida G.; Postiglione, Lorena; Salzano, Davide; Grierson, Claire S.; Marucci, Lucia; Savery, Nigel J.; di Bernardo, Mario;
Open Access
Abstract
<p><strong>Abstract</strong></p> <p>We study both&nbsp;<em>in silico</em>&nbsp;and&nbsp;<em>in vivo</em>&nbsp;the real-time feedback control of a molecular titration motif that has been earmarked as a fundamental component of antithetic and multicellular feedback control schemes in&nbsp;<em>E.&nbsp;coli</em>. We show that an external feedback control strategy can successfully regulate the average fluorescence output of a bacterial cell population to a desired constant level in real-time. We also provide&nbsp;<em>in silico</em>&nbsp;evidence that the same strategy can be used to track a time-varying reference signal where the set-point is switched to a different...
Persistent Identifiers
Funded by
EC| COSY-BIO
Project
COSY-BIO
Control Engineering of Biological Systems for Reliable Synthetic Biology Applications
  • Funder: European Commission (EC)
  • Project Code: 766840
  • Funding stream: H2020
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