publication . Preprint . Article . Other literature type . 2017

LIN28 selectively modulates a subclass of let-7 microRNAs

Dmytro Ustianenko; Hua-Sheng Chiu; Sebastien M. Weyn-Vanhentenryck; Pavel Sumazin; Chaolin Zhang;
Open Access English
  • Published: 12 Oct 2017
  • Publisher: Cold Spring Harbor Laboratory
Abstract
<jats:title>Abstract</jats:title><jats:p>LIN28 is a bipartite RNA-binding protein that post-transcriptionally inhibits let-7 microRNAs to regulate development and influence disease states. However, the mechanisms of let-7 suppression remains poorly understood, because LIN28 recognition depends on coordinated targeting by both the zinc knuckle domain (ZKD)—which binds a GGAG-like element in the precursor—and the cold shock domain (CSD), whose binding sites have not been systematically characterized. By leveraging single-nucleotide-resolution mapping of LIN28 binding sites <jats:italic>in vivo</jats:italic>, we determined that the CSD recognizes a (U)GAU motif. Th...
Subjects
free text keywords: let-7 microRNA biogenesis, cold shock domani, bipartite binding, selective suppression, Cold-shock domain, Genetics, Subclass, Binding site, Cell biology, LIN28, Biology, In vivo, microRNA, Molecular Biology, Biogenesis, Article
Funded by
EC| PrECISE
Project
PrECISE
PERSONALIZED ENGINE FOR CANCER INTEGRATIVE STUDY AND EVALUATION
  • Funder: European Commission (EC)
  • Project Code: 668858
  • Funding stream: H2020 | RIA
,
EC| moreRNA
Project
moreRNA
Modification and regulation of coding and non-coding RNA pathways
  • Funder: European Commission (EC)
  • Project Code: 682291
  • Funding stream: H2020 | ERC | ERC-COG
,
NIH| High-performance compute cluster for biomedical computing
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 1S10OD012351-01
  • Funding stream: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
,
NIH| CLIP Tool Kit (CTK): pipeline, user interface and tutorials for CLIP data analysis
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 1R03HG009528-01
  • Funding stream: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
,
NIH| RNA regulatory networks in motor neuron development and function
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 3R01NS089676-03S1
  • Funding stream: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
Abstract
<jats:title>Abstract</jats:title><jats:p>LIN28 is a bipartite RNA-binding protein that post-transcriptionally inhibits let-7 microRNAs to regulate development and influence disease states. However, the mechanisms of let-7 suppression remains poorly understood, because LIN28 recognition depends on coordinated targeting by both the zinc knuckle domain (ZKD)—which binds a GGAG-like element in the precursor—and the cold shock domain (CSD), whose binding sites have not been systematically characterized. By leveraging single-nucleotide-resolution mapping of LIN28 binding sites <jats:italic>in vivo</jats:italic>, we determined that the CSD recognizes a (U)GAU motif. Th...
Subjects
free text keywords: let-7 microRNA biogenesis, cold shock domani, bipartite binding, selective suppression, Cold-shock domain, Genetics, Subclass, Binding site, Cell biology, LIN28, Biology, In vivo, microRNA, Molecular Biology, Biogenesis, Article
Funded by
EC| PrECISE
Project
PrECISE
PERSONALIZED ENGINE FOR CANCER INTEGRATIVE STUDY AND EVALUATION
  • Funder: European Commission (EC)
  • Project Code: 668858
  • Funding stream: H2020 | RIA
,
EC| moreRNA
Project
moreRNA
Modification and regulation of coding and non-coding RNA pathways
  • Funder: European Commission (EC)
  • Project Code: 682291
  • Funding stream: H2020 | ERC | ERC-COG
,
NIH| High-performance compute cluster for biomedical computing
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 1S10OD012351-01
  • Funding stream: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
,
NIH| CLIP Tool Kit (CTK): pipeline, user interface and tutorials for CLIP data analysis
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 1R03HG009528-01
  • Funding stream: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
,
NIH| RNA regulatory networks in motor neuron development and function
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 3R01NS089676-03S1
  • Funding stream: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
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