publication . Article . Other literature type . 2015

G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

Iain C Macaulay; Wilfried Haerty; Parveen Kumar; Yang I Li; Tim Xiaoming Hu; Mabel J Teng; Mubeen Goolam; Nathalie Saurat; Paul Coupland; Lesley M Shirley; ...
Open Access
  • Published: 27 Apr 2015
  • Country: United States
Abstract
The ability to simultaneously sequence the genome and transcriptome of the same single cell offers a powerful means to dissect functional genetic heterogeneity at the cellular level. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells we reveal cellular properties that cannot be inferred from DNA or RNA sequencing alone, including associations between DNA copy number variation and gene expression dosage. We further demonstrate the detection of coding interchromosomal fusions and single nucleotide variants in both the genomes and transcriptomes of individ...
Subjects
free text keywords: Illumina dye sequencing, Genetics, Exome sequencing, Cancer genome sequencing, Single cell sequencing, Bioinformatics, Genomics, Nucleic acid amplification technique, Genome, Molecular biology, Biology, Massive parallel sequencing
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publication . Article . Other literature type . 2015

G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

Iain C Macaulay; Wilfried Haerty; Parveen Kumar; Yang I Li; Tim Xiaoming Hu; Mabel J Teng; Mubeen Goolam; Nathalie Saurat; Paul Coupland; Lesley M Shirley; ...