publication . Other literature type . Article . 2014

Long Term Storage Of Bacterial Strains

Open Access
  • Published: 30 Dec 2014
  • Publisher: Zenodo
**Time required:** Cells are grown 6 hours to overnight; a total of less than 5 minutes bench time for each strain. ### Procedure: **Day 1** 1. Inoculate a 15 ml culture tube containing 5 ml of LBM or LBM+antibiotic selective medium with a freshly grown isolated colony. Incubate at 37 degrees C until culture is in late log or stationary phase (usually 5 hours to overnight). **Day 2** 1. For each strain to be stored at -80 degrees C for the archives prepare a sterile labeled cryovial. Pipet 225 ul sterile 80% glycerol into the cryovial. Add 1.0 ml of the bacterial culture (frozen stock will be 15% glycerol). Mix well (vortex) and place tube at -80 degrees C. - Fo...
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Other literature type . 2014
Provider: Datacite
Article . 2014
Provider: ZENODO
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