publication . Other literature type . Article . 2014

Long Term Storage Of Bacterial Strains

sprotocols;
Open Access
  • Published: 30 Dec 2014
  • Publisher: Zenodo
Abstract
**Time required:** Cells are grown 6 hours to overnight; a total of less than 5 minutes bench time for each strain. ### Procedure: **Day 1** 1. Inoculate a 15 ml culture tube containing 5 ml of LBM or LBM+antibiotic selective medium with a freshly grown isolated colony. Incubate at 37 degrees C until culture is in late log or stationary phase (usually 5 hours to overnight). **Day 2** 1. For each strain to be stored at -80 degrees C for the archives prepare a sterile labeled cryovial. Pipet 225 ul sterile 80% glycerol into the cryovial. Add 1.0 ml of the bacterial culture (frozen stock will be 15% glycerol). Mix well (vortex) and place tube at -80 degrees C. - Fo...
Persistent Identifiers
Download fromView all 2 versions
Zenodo
Other literature type . 2014
Provider: Datacite
ZENODO
Article . 2014
Provider: ZENODO
Any information missing or wrong?Report an Issue