Stainless Steel Sponge as a Carrier for Immobilization of Rhizopus nigricans Isolate used for Biotrnsformation of Progesterone to 11α-hydroxprogesterone
Essam. M. Ahmed
Biotransformation, Rhizopus nigricans, 11α- hydroxyprogesterone
Biotransformation is an important tool to provide the intermediate for steroid manufacturing. Stainless steel sponge was used as a carrier to immobilize an isolate of Rhizopus nigricans which can convert progesterone to 11α-hydroxyprogesterone (11α-HP). The conversion ability of the isolate was measured. The immobilized cells showed the ability to withstand the stress of the biotransformation process. Different parameters were studied to achieve the optimum conditions for 11α-HP production. The immobilized cells were reused for seven successive runs and maintained high bioconversion abilities during the first two runs then lost the conversion ability in seventh run.