publication . Article . 2011

RT-PCR/electrospray ionization mass spectrometry approach in detection and characterization of influenza viruses.

Deyde, Varough M.; Sampath, Rangarajan; Gubareva, Larisa V.;
Open Access
  • Published: 01 Jan 2011 Journal: Expert Review of Molecular Diagnostics, volume 11, pages 41-52 (issn: 1473-7159, eissn: 1744-8352, Copyright policy)
  • Publisher: Informa UK Limited
Reverse-transcription PCR (RT-PCR) coupled with electrospray ionization mass spectrometry (ESI-MS) is a high-throughput nucleic acid-based technology that relies on the accurate measurement of the molecular weight of PCR amplicons that can be used to deduce the base counts (number of As, Gs, Cs and Ts) of DNA. These amplicons represent highly variable regions with information-rich sequences, which are flanked by broad-range primers designed based on highly conserved loci. This technology was first introduced in 2005 for microbial identification and subtyping, and was later applied to influenza virus detection and identification. The influenza RT-PCR/ESI-MS assay...
free text keywords: Pathology and Forensic Medicine, Molecular Medicine, Genetics, Molecular Biology, Electrospray ionization, Subtyping, Nucleic acid, Bioinformatics, Virus, Primer (molecular biology), Real-time polymerase chain reaction, Biology, Amplicon, Mass spectrometry
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