publication . Article . 2016

Intracerebral transplantation of interleukin 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse model

Marleen Verhoye; Peter Ponsaerts; Dearbhaile Dooley; Caroline Guglielmetti; Annemie Van der Linden; Alessandra Quarta; Zwi N. Berneman; Jelle Praet; Evi Lemmens; Eva Santermans; ...
Open Access English
  • Published: 09 Nov 2016
  • Country: Belgium
Background: Promoting the neuroprotective and repair-inducing effector functions of microglia and macrophages, by means of M2 polarisation or alternative activation, is expected to become a new therapeutic approach for central nervous system (CNS) disorders in which detrimental pro-inflammatory microglia and/or macrophages display a major contribution to the neuropathology. In this study, we present a novel in vivo approach using intracerebral grafting of mesenchymal stem cells (MSC) genetically engineered to secrete interleukin 13 (IL13-MSC). Methods: In the first experimental setup, control MSC and IL13-MSC were grafted in the CNS of eGFP(+) bone marrow chimae...
free text keywords: Interleukin 13; Mesenchymal stem cells; Neuroinflammation; Transplantation; Magnetic resonance imaging, Research, Interleukin 13, Mesenchymal stem cells, Neuroinflammation, Transplantation, Magnetic resonance imaging, Immunology, Cellular and Molecular Neuroscience, Neurology, Human medicine, Gliosis, medicine.symptom, medicine, Cancer research, Neuroprotection, Oligodendrocyte, medicine.anatomical_structure, Biology, Mesenchymal stem cell, Microglia, Neuroscience, Microgliosis
Funded by
Imaging of Neuroinflammation in Neurodegenerative Diseases
  • Funder: European Commission (EC)
  • Project Code: 278850
  • Funding stream: FP7 | SP1 | HEALTH
39 references, page 1 of 3

Benakis, C, Garcia-Bonilla, L, Iadecola, C, Anrather, J. The role of microglia and myeloid immune cells in acute cerebral ischemia. Front Cell Neurosci. 2014; 8: 461 [OpenAIRE] [PubMed]

Morganti, JM, Jopson, TD, Liu, S, Riparip, LK, Guandique, CK, Gupta, N, Ferguson, AR, Rosi, S. CCR2 antagonism alters brain macrophage polarization and ameliorates cognitive dysfunction induced by traumatic brain injury. J Neurosci. 2015; 35: 748-760 [OpenAIRE] [PubMed] [DOI]

Yamasaki, R, Lu, H, Butovsky, O, Ohno, N, Rietsch, AM, Cialic, R, Wu, PM, Doykan, CE, Lin, J, Cotleur, AC. Differential roles of microglia and monocytes in the inflamed central nervous system. J Exp Med. 2014; 211: 1533-1549 [OpenAIRE] [PubMed] [DOI]

Zhou, X, He, X, Ren, Y. Function of microglia and macrophages in secondary damage after spinal cord injury. Neural Regen Res. 2014; 9: 1787-1795 [OpenAIRE] [PubMed] [DOI]

Shechter, R, Schwartz, M. Harnessing monocyte-derived macrophages to control central nervous system pathologies: no longer ‘if’ but ‘how’. J Pathol. 2013; 229: 332-346 [OpenAIRE] [PubMed] [DOI]

Murray, PJ, Allen, JE, Biswas, SK, Fisher, EA, Gilroy, DW, Goerdt, S, Gordon, S, Hamilton, JA, Ivashkiv, LB, Lawrence, T. Macrophage activation and polarization: nomenclature and experimental guidelines. Immunity. 2014; 41: 14-20 [OpenAIRE] [PubMed] [DOI]

Van Dyken, SJ, Locksley, RM. Interleukin-4- and interleukin-13-mediated alternatively activated macrophages: roles in homeostasis and disease. Annu Rev Immunol. 2013; 31: 317-343 [OpenAIRE] [PubMed] [DOI]

Ibraheem, D, Elaissari, A, Fessi, H. Gene therapy and DNA delivery systems. Int J Pharm. 2014; 459: 70-83 [OpenAIRE] [PubMed] [DOI]

De Vocht, N, Praet, J, Reekmans, K, Le Blon, D, Hoornaert, C, Daans, J, Berneman, Z, Van der Linden, A, Ponsaerts, P. Tackling the physiological barriers for successful mesenchymal stem cell transplantation into the central nervous system. Stem Cell Res Ther. 2013; 4: 101 [OpenAIRE] [PubMed] [DOI]

Bergwerf, I, Tambuyzer, B, De Vocht, N, Reekmans, K, Praet, J, Daans, J, Chatterjee, S, Pauwels, P, Van der Linden, A, Berneman, ZN, Ponsaerts, P. Recognition of cellular implants by the brain’s innate immune system. Immunol Cell Biol. 2011; 89: 511-516 [OpenAIRE] [PubMed] [DOI]

Costa, R, Bergwerf, I, Santermans, E, De Vocht, N, Praet, J, Daans, J, Le Blon, D, Hoornaert, C, Reekmans, K, Hens, N. Distinct in vitro properties of embryonic and extraembryonic fibroblast-like cells are reflected in their in vivo behavior following grafting in the adult mouse brain. Cell Transplant. 2015; 24: 223-233 [OpenAIRE] [PubMed] [DOI]

De Vocht, N, Lin, D, Praet, J, Hoornaert, C, Reekmans, K, Le Blon, D, Daans, J, Pauwels, P, Goossens, H, Hens, N. Quantitative and phenotypic analysis of mesenchymal stromal cell graft survival and recognition by microglia and astrocytes in mouse brain. Immunobiology. 2013; 218: 696-705 [OpenAIRE] [PubMed] [DOI]

Le Blon, D, Hoornaert, C, Daans, J, Santermans, E, Hens, N, Goossens, H, Berneman, Z, Ponsaerts, P. Distinct spatial distribution of microglia and macrophages following mesenchymal stem cell implantation in mouse brain. Immunol Cell Biol. 2014; 92: 650-658 [OpenAIRE] [PubMed] [DOI]

Praet, J, Reekmans, K, Lin, D, De Vocht, N, Bergwerf, I, Tambuyzer, B, Daans, J, Hens, N, Goossens, H, Pauwels, P. Cell type-associated differences in migration, survival, and immunogenicity following grafting in CNS tissue. Cell Transplant. 2012; 21: 1867-1881 [OpenAIRE] [PubMed] [DOI]

15.Praet J, Santermans E, Daans J, Le Blon D, Hoornaert C, Goossens H, Hens N, Van der Linden A, Berneman Z, Ponsaerts P. Early inflammatory responses following cell grafting in the CNS trigger activation of the sub-ventricular zone: a proposed model of sequential cellular events. Cell Transplant. 2014;24(8):1481–92.

39 references, page 1 of 3
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