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Automatic synchronisation of the cell cycle in budding yeast through closed-loop feedback control

Perrino, Giansimone; Napolitano, Sara; Galdi, Francesca; La Regina, Antonella; Fiore, Davide; Giuliano, Teresa; di Bernardo, Mario; di Bernardo, Diego;
Open Access
Abstract
<p>Abstract</p> <p>The cell cycle is the process by which eukaryotic cells replicate. Yeast cells cycle asynchronously with each cell in the population budding at a different time. Although there are several experimental approaches to synchronise cells, these usually work only in the short-term. Here, we build a cyber-genetic system to achieve long-term synchronisation of the cell population, by interfacing genetically modified yeast cells with a computer by means of microfluidics to dynamically change medium, and a microscope to estimate cell cycle phases of individual cells. The computer implements a controller algorithm to decide when, and for how long, to c...
Persistent Identifiers
Funded by
EC| COSY-BIO
Project
COSY-BIO
Control Engineering of Biological Systems for Reliable Synthetic Biology Applications
  • Funder: European Commission (EC)
  • Project Code: 766840
  • Funding stream: H2020
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