publication . Article . 1987

Ethidium bromide: Destruction and decontamination of solutions

George Lunn; Eric B. Sansone;
Open Access
  • Published: 01 May 1987 Journal: Analytical Biochemistry, volume 162, pages 453-458 (issn: 0003-2697, Copyright policy)
  • Publisher: Elsevier BV
Abstract
Abstract Ethidium bromide in water, TBE buffer, Mops buffer, and cesium chloride solution may be completely degraded by reaction with sodium nitrite and hypophosphorous acid. Only nonmutagenic reaction mixtures were produced. Destruction was >99.8% in all cases; the limit of detection was 0.5 μg ethidium bromide per milliliter of solution. Ethidium bromide also may be removed completely from the above solutions by using Amberlite XAD-16 resin. The limit of detection was 0.05 μg ethidium bromide per milliliter of solution (0.27 μg/ml when cesium chloride solution was used).
Persistent Identifiers
Subjects
free text keywords: Chloride, medicine.drug, medicine, Nuclear chemistry, Detection limit, Inorganic chemistry, Hypophosphorous acid, chemistry.chemical_compound, chemistry, Sodium nitrite, MOPS, Ethidium bromide, TBE buffer, Amberlite
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